| A novel motif in HIV-1 Nef that regulates MIP-1beta chemokine release in macrophages. | |
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MedLine Citation:
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PMID: 20504918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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All primate lentiviruses encode Nef, an accessory protein that is important for viral pathogenicity in vivo. Lentiviral Nef proteins regulate the release of chemokines (MIP-1 alpha/beta) from infected macrophages, thereby enhancing virus dissemination (S. Swingler, A. Mann, J. Jacque, B. Brichacek, V. G. Sasseville, K. Williams, A. A. Lackner, E. N. Janoff, R. Wang, D. Fisher, and M. Stevenson, Nat. Med. 5:997-1003, 1999). In the current study, we have identified a novel domain within Nef (K(92)EK) that is required for Nef-dependent MIP-1beta production by infected macrophages. Mutations in this domain abrogated MIP-1beta induction but did not affect other Nef-ascribed activities, such as CD4 or major histocompatibility complex (MHC) class Iota downregulation. This further underscores Nef as a modular protein with genetically separable activities that may contribute to its role in viral replication and pathogenicity. |
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Authors:
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Lue Dai; Mario Stevenson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-05-26 |
Journal Detail:
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Title: Journal of virology Volume: 84 ISSN: 1098-5514 ISO Abbreviation: J. Virol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-19 Completed Date: 2010-08-06 Revised Date: 2011-07-22 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 8327-31 Citation Subset: IM |
Affiliation:
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University of Massachusetts Medical School, Program in Molecular Medicine, 373 Plantation Street, Worcester, Massachusetts 01605, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Chemokine CCL4 / secretion* HIV-1 / immunology*, pathogenicity* Humans Macrophages / immunology*, virology* Molecular Sequence Data Mutation, Missense Protein Structure, Tertiary Virulence Factors / genetics, physiology* nef Gene Products, Human Immunodeficiency Virus / genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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P30-AI42845/AI/NIAID NIH HHS; R01-AI032890/AI/NIAID NIH HHS; R01-MH64411/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL4; 0/Virulence Factors; 0/nef Gene Products, Human Immunodeficiency Virus; 0/nef protein, Human immunodeficiency virus 1 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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