Document Detail


A novel monoclonal antibody that recognizes apical membrane of frog taste cells.
MedLine Citation:
PMID:  9915117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We established a hybridoma clone 1N1 that produced a monoclonal antibody to stain the apical portion of frog taste cells, by directly immunizing taste discs of the bullfrog (Rana catesbeiana) without any dispersion procedure of the taste organ. The antibody stained discrete regions on the surface of the taste discs, but did not stain the epithelium sheet of the tongue devoid of taste discs. The antibody stained approximately 93% of the taste discs tested (172/184) derived from nine frogs, showing that distribution of the antigen was common to most of the taste discs. The following observations strongly suggested that the antibody recognized a certain antigen on the apical membrane of the taste cells. (i) The antibody selectively stained cross points of intermucus areas on the surface of the taste disc. Neither the mucus cells nor the wing cells that mainly cover the surface were stained with the antibody. (ii) Dispersed taste cells were prepared by calcium ion chelating and subsequently by collagenase treatment to avoid digestion of the antigen. The antibody stained the apical end of the taste cells.
Authors:
N Higashi; K Tsujii; J Sunamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemical senses     Volume:  23     ISSN:  0379-864X     ISO Abbreviation:  Chem. Senses     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-03-30     Completed Date:  1999-03-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8217190     Medline TA:  Chem Senses     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  709-16     Citation Subset:  IM    
Affiliation:
Kao Institute for Fundamental Research, Kao Corporation, Tokyo, Japan. higashi@mol.f.u-tokyo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / metabolism*
Antibody Formation
Cell Membrane / metabolism
Hybridomas / immunology
Immunohistochemistry
Rana catesbeiana
Staining and Labeling / methods
Taste Buds / cytology*,  metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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