Document Detail


A novel monoclonal antibody against murine IL-2 receptor beta-chain. Characterization of receptor expression in normal lymphoid cells and EL-4 cells.
MedLine Citation:
PMID:  1918958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mAb specific for the murine IL-2R beta-chain (IL-2R beta) was produced by immunizing a rat with a rat transfectant cell line expressing a large number of cDNA-encoded murine IL-2R beta. The mAb, designated TM-beta 1, is specifically reactive with the murine IL-2R beta cDNA-transfectant but not with the recipient cell, and immunoprecipitates murine IL-2R beta of Mr 75 to 85 kDa. TM-beta 1 mAb completely abolished the high affinity IL-2 binding by inhibiting the ligand binding to IL-2R beta. Murine IL-2R beta was found to be constitutively expressed on a subpopulation of CD8+ T cells and almost all NK1.1+ NK cells in the spleen, whereas TM-beta 1 mAb inhibited the proliferation of spleen cells induced by 1 nM of IL-2. Interestingly, EL-4 cells that express murine IL-2R beta as detected by TM-beta 1 mAb can bind neither human nor murine IL-2 under the intermediate affinity conditions, although cDNA-directed human IL-2R beta expressed in the same EL-4 cells has been previously shown to manifest the intermediate affinity IL-2 binding. These results may imply that functional expression of IL-2R beta is differentially regulated between humans and mice. Finally, our neutralizing anti-IL-2R beta mAb TM-beta 1 will be useful not only for various in vitro studies but also for in vivo studies to directly investigate the possible involvement of the IL-2/IL-2R pathway in the generation and differentiation of T lymphocytes and NK cells.
Authors:
T Tanaka; M Tsudo; H Karasuyama; F Kitamura; T Kono; M Hatakeyama; T Taniguchi; M Miyasaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  147     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1991-10-28     Completed Date:  1991-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2222-8     Citation Subset:  AIM; IM    
Affiliation:
Department of Immunology, Tokyo Metropolitan Institute of Medical Science, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use*,  immunology
Cell Division / drug effects
Cell Line
Female
Interleukin-2 / metabolism,  pharmacology
Lymphocytes / chemistry*
Lymphoma / metabolism
Mice
Mice, Inbred C57BL
Rats
Rats, Inbred Strains
Receptors, Interleukin-2 / analysis*,  immunology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Interleukin-2; 0/Receptors, Interleukin-2

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