Document Detail

A novel model of accelerated intimal hyperplasia in the pig iliac artery.
MedLine Citation:
PMID:  22050434     Owner:  NLM     Status:  MEDLINE    
There is no good animal model of large artery injury-induced intimal hyperplasia (IH). Those available are reproducible, providing only a few layers of proliferating cells or have the disadvantage of the presence of a metallic stent that complicates histology evaluation. This study was designed to develop a new, simple model of accelerated IH based on balloon injury in conjunction with disruption of the Internal Elastic Lamina (IEL) in pig external iliac arteries. Iliac artery injury (n = 24) was performed in 12 Yorkshire pigs divided in two groups: Group I (n = 10), overdistention injury induced by an oversized non-compliant balloon; Group II (n = 14), arterial wall disruption by pulling back an isometric cutting balloon (CB) followed by stretching with a compliant Fogarty Balloon (FB). At two weeks, arteries were processed for morphometric analysis and immunohistochemistry (IHC) for smooth muscle cells (SMC) and proliferating cell nuclear antigen (PCNA). When comparing the two groups, at 2 weeks, arteries of group II had a higher incidence of IH (100%vs. 50%, P = 0.0059), increased intimal areas (2.54 ± 0.33 mm(2) vs. 0.93 ± 0.36 mm(2) , P = 0.004), increased intimal area/Media area ratios (0.95 ± 0.1 vs. 0.28 ± 0.05; P < 0.0001) and decreased lumen areas (6.24 ± 0.44 vs. 9.48 ± 1.56, P = 0.026). No thrombosis was noticed in Group II. Neointima was composed by proliferating SMC located with the highest concentration in the area of IEL disruption (IHC). Arterial injury by pulling back CB and FB induces significant IH in pig iliac arteries by two weeks without thrombosis. This model is superior to the classical overdistention non-compliant model and should be useful and cost-effective for preclinical testing of procedures designed to inhibit IH in large peripheral arteries.
Rabih Houbballah; Alessandro Robaldo; Hassan Albadawi; James Titus; Glenn M LaMuraglia
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Publication Detail:
Type:  Journal Article     Date:  2011-11-03
Journal Detail:
Title:  International journal of experimental pathology     Volume:  92     ISSN:  1365-2613     ISO Abbreviation:  Int J Exp Pathol     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-29     Completed Date:  2012-01-24     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9014042     Medline TA:  Int J Exp Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  422-7     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. International Journal of Experimental Pathology © 2011 International Journal of Experimental Pathology.
Division of Vascular and Endovascular Surgery of the General Surgical Services, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
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MeSH Terms
Catheterization / adverse effects*
Cell Proliferation
Disease Models, Animal*
Hyperplasia / etiology,  pathology
Iliac Artery / metabolism,  pathology*
Myocytes, Smooth Muscle / metabolism,  pathology
Neointima / metabolism,  pathology
Proliferating Cell Nuclear Antigen / metabolism
Time Factors
Tunica Intima / metabolism,  pathology*
Tunica Media / metabolism,  pathology
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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