| A novel mitosis-specific dynamic actin structure in Dictyostelium cells. | |
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MedLine Citation:
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PMID: 18029399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell division of various animal cells depends on their attachment to a substratum. Dictyostelium cells deficient in type II myosin, analogous to myosin in muscle, can divide on a substratum without the contractile ring. To investigate the mechanism of this substratum-dependent cytokinesis, the dynamics of actin in the ventral cortex were observed by confocal and total internal reflection fluorescence microscopy. Specifically during mitosis, we found novel actin-containing structures (mitosis-specific dynamic actin structures, MiDASes) underneath the nuclei and centrosomes. When the nucleus divided, the MiDAS also split in two and followed the movement of the daughter nuclei. At that time, the distal ends of astral microtubules reached mainly the MiDAS regions of the ventral cortex. An inhibitor of microtubules induced disappearance of MiDASes, leading to aborted cytokinesis, suggesting that astral microtubules are required for the formation and maintenance of MiDASes. Fluorescence recovery after photobleaching experiments revealed that the MiDAS was highly dynamic and comprised small actin-containing dot-like structures. Interference reflection microscopy and assays blowing away the cell bodies by jet streaming showed that MiDASes were major attachment sites of dividing cells. Thus, the MiDASes are strong candidates for scaffolds for substratum-dependent cytokinesis, serving to transmit mechanical force to the substratum. |
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Authors:
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Go Itoh; Shigehiko Yumura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-20 |
Journal Detail:
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Title: Journal of cell science Volume: 120 ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-06 Completed Date: 2008-03-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: England |
Other Details:
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Languages: eng Pagination: 4302-9 Citation Subset: IM |
Affiliation:
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Department of Biology, Faculty of Science, Yamaguchi University, Yamaguchi 753-8512, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism*,
ultrastructure Animals Cell Nucleus / metabolism Centrosome / metabolism Cytokinesis* Cytoskeleton / metabolism* Dictyostelium / cytology*, metabolism*, ultrastructure Fluorescence Recovery After Photobleaching Green Fluorescent Proteins Microfilament Proteins / metabolism Microtubules / metabolism Mitosis* Myosin Type II / metabolism* Protozoan Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Microfilament Proteins; 0/Protozoan Proteins; 147336-22-9/Green Fluorescent Proteins; EC 3.6.1.-/Myosin Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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