| A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells. | |
MedLine Citation:
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PMID: 16874368 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The importance of research involving non-adherent cell lines, primary cells and blood cells is generally undisputed. However, the task of investigating the complexity and heterogeneity of these cells calls for their long-run monitoring at a single-cell resolution. Such a capability is currently unavailable without having to use disruptive cell tethering. The present Cell Retainer (CR) concept enables high-content correlative multi-parametric measurements, from the functional to molecular level, of the same living individual non-adherent cells within a population. Thereby, despite extensive long-term bio-manipulations, the cells preserve their identity without tethering. Several exemplary experiments, using a microscope-slide-based version of the CR, are presented, which could not be performed by other state of the art methods. |
Authors:
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Mordechai Deutsch; Assaf Deutsch; Orian Shirihai; Ihar Hurevich; Elena Afrimzon; Yana Shafran; Naomi Zurgil |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-02 |
Journal Detail:
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Title: Lab on a chip Volume: 6 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-07-28 Completed Date: 2007-05-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 995-1000 Citation Subset: IM |
Affiliation:
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Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan 52900, Israel. rtcellom@mail.biu.ac.il |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blood Cells
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cytology* Cell Culture Techniques* Humans Microfluidic Analytical Techniques* Microfluidics* / instrumentation U937 Cells |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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