Document Detail


A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells.
MedLine Citation:
PMID:  16874368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The importance of research involving non-adherent cell lines, primary cells and blood cells is generally undisputed. However, the task of investigating the complexity and heterogeneity of these cells calls for their long-run monitoring at a single-cell resolution. Such a capability is currently unavailable without having to use disruptive cell tethering. The present Cell Retainer (CR) concept enables high-content correlative multi-parametric measurements, from the functional to molecular level, of the same living individual non-adherent cells within a population. Thereby, despite extensive long-term bio-manipulations, the cells preserve their identity without tethering. Several exemplary experiments, using a microscope-slide-based version of the CR, are presented, which could not be performed by other state of the art methods.
Authors:
Mordechai Deutsch; Assaf Deutsch; Orian Shirihai; Ihar Hurevich; Elena Afrimzon; Yana Shafran; Naomi Zurgil
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-02
Journal Detail:
Title:  Lab on a chip     Volume:  6     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-28     Completed Date:  2007-05-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  995-1000     Citation Subset:  IM    
Affiliation:
Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan 52900, Israel. rtcellom@mail.biu.ac.il
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MeSH Terms
Descriptor/Qualifier:
Blood Cells / cytology*
Cell Culture Techniques*
Humans
Microfluidic Analytical Techniques*
Microfluidics* / instrumentation
U937 Cells

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