| A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells. | |
MedLine Citation:
|
PMID: 16874368 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The importance of research involving non-adherent cell lines, primary cells and blood cells is generally undisputed. However, the task of investigating the complexity and heterogeneity of these cells calls for their long-run monitoring at a single-cell resolution. Such a capability is currently unavailable without having to use disruptive cell tethering. The present Cell Retainer (CR) concept enables high-content correlative multi-parametric measurements, from the functional to molecular level, of the same living individual non-adherent cells within a population. Thereby, despite extensive long-term bio-manipulations, the cells preserve their identity without tethering. Several exemplary experiments, using a microscope-slide-based version of the CR, are presented, which could not be performed by other state of the art methods. |
Authors:
|
Mordechai Deutsch; Assaf Deutsch; Orian Shirihai; Ihar Hurevich; Elena Afrimzon; Yana Shafran; Naomi Zurgil |
Related Documents
:
|
20812258 - Modulation of viability of live cells by focused ion-beam exposure. 16618928 - Designing a nano-interface in a microfluidic chip to probe living cells: challenges and... 19067748 - Toward minimal bacterial cells: evolution vs. design. 16471438 - Optical and acoustic detection of laser-generated microbubbles in single cells. 25263458 - The interplay between cell death signaling pathways in the heart. 21461878 - Activation of protease-activated receptor (par) 1 by frog trefoil factor (tff) 2 and pa... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-02 |
Journal Detail:
|
Title: Lab on a chip Volume: 6 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2006 Aug |
Date Detail:
|
Created Date: 2006-07-28 Completed Date: 2007-05-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
|
Languages: eng Pagination: 995-1000 Citation Subset: IM |
Affiliation:
|
Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar Ilan University, Ramat Gan 52900, Israel. rtcellom@mail.biu.ac.il |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blood Cells
/
cytology* Cell Culture Techniques* Humans Microfluidic Analytical Techniques* Microfluidics* / instrumentation U937 Cells |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pumpless, selective docking of yeast cells inside a microfluidic channel induced by receding meniscu...
Next Document: Microchannel device using self-spreading lipid bilayer as molecule carrier.