Document Detail


A novel methodology for the establishment of neuroblastoma cell lines from metastatic marrow. Expression of surface markers, neurofilaments, MDR-1 and myc proteins.
MedLine Citation:
PMID:  1348778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A methodology for rapid isolation of neuroblastoma (NB) cells from marrow with metastatic NB cells was developed using a cocktail of five antibodies and magnetic microspheres coated with secondary antibodies. Cells bound to microspheres were released by brief exposure to chymopapain, followed by repeated culture of released cells in serum supplemented DMEM medium and selection for adherent cells. Using this methodology, over 35 primary cell lines were obtained free of contaminating normal cells. Detailed analyses of over 14 cell lines revealed gross differences in cell phenotype, size, morphology development of neurite processes, and doubling time (40h-80 h). All cell lines expressed the 145 kDa neurofilament (NF) and a few expressed the 200 kDa NF, with very little or no expression of the 68 kDa NF. DNA analyses revealed 80% near diploid cell lines. High expression of the MDR-1 protein was detected in 6/22 of the cell lines tested.
Authors:
Y Gazitt; Y J He; J Graham-Pole
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunological methods     Volume:  148     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-05-19     Completed Date:  1992-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  171-8     Citation Subset:  IM    
Affiliation:
Department of Pediatrics Hematology Oncology, University of Florida Medical Center, Gainesville 32610.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Neoplasm
Bone Marrow Purging / methods
Bone Neoplasms / metabolism*,  secondary
Cell Line, Transformed
DNA / analysis
Flow Cytometry
Fluorescent Antibody Technique
Gene Expression*
Humans
Membrane Glycoproteins / biosynthesis*
Neuroblastoma / metabolism*
Neurofilament Proteins / biosynthesis*
P-Glycoprotein
Ploidies
Proto-Oncogene Proteins c-myc / biosynthesis*
Tumor Markers, Biological*
Chemical
Reg. No./Substance:
0/Antibodies, Neoplasm; 0/Membrane Glycoproteins; 0/Neurofilament Proteins; 0/P-Glycoprotein; 0/Proto-Oncogene Proteins c-myc; 0/Tumor Markers, Biological; 9007-49-2/DNA

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