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A novel method to isolate cells with conditional gene expression using fluorescence activated cell sorting.
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MedLine Citation:
PMID:  9365269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An inducible expression system using control elements of the tetracycline resistance operon has recently shown promise for conditional gene expression of any gene of interest. However, intensive screening of multiple independent clones is often required to find cell lines with optimal induction characteristics. By coupling expression of the gene of interest with a fluorescent marker, we have developed a novel fluorescence activated cell sorting (FACS) based strategy to isolate cells with desirable expression characteristics, thus alleviating the laborious isolation and analysis of multiple independent clones.
Authors:
K M Klucher; M J Gerlach; G Q Daley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  25     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-01-20     Completed Date:  1998-01-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4858-60     Citation Subset:  IM    
Affiliation:
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods*
Flow Cytometry / methods*
Fusion Proteins, bcr-abl / genetics*
Gene Expression Regulation*
Humans
Mice
Operon
Tetracycline Resistance / genetics
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Fusion Proteins, bcr-abl
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 1 Month: 12 Year: 1997
Volume: 25 Issue: 23
First Page: 4858 Last Page: 4860
ID: 147116
PubMed Id: 9365269
Publisher Item Identifier: gka769

A novel method to isolate cells with conditional gene expression using fluorescence activated cell sorting.
K M Klucher
M J Gerlach
G Q Daley
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.


Article Categories:
  • Research Article


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