Document Detail

A novel method for the reduction of numbers of Listeria monocytogenes cells by freezing in combination with an essential oil in bacteriological media.
MedLine Citation:
PMID:  12636290     Owner:  NLM     Status:  MEDLINE    
The use of multiple freeze (-20 degrees C)-thaw cycles in combination with isoeugenol and polysorbate 80 was investigated as a method for the reduction of numbers of Listeria monocytogenes cells in a bacteriological medium. Three freeze (1 h, -20 degrees C)-thaw cycles in the presence of isoeugenol at concentrations of 0, 100, and 300 ppm resulted in average L. monocytogenes reductions of 0.69, 2.65, and 3.3 log10 MPN (most probable number) per ml, respectively. Increasing the number of freeze-thaw cycles further decreased cell numbers, with reductions of nearly 5 log10 MPN/ml being obtained with six freeze-thaw cycles. Freeze-thaw cycles were effective in reducing cell numbers at isoeugenol concentrations down to 25 ppm. Rapid freezing rates with liquid nitrogen were found to be less effective in reducing numbers of L. monocytogenes cells. Two rapid freeze-thaw cycles in the presence of 100 ppm isoeugenol and polysorbate 80 resulted in a reduction of 1.45 log10 MPN/ml. Two freezing (-20 degrees C) cycles involving slow freezing and thawing rates with samples being held frozen for 6 h for each cycle resulted in reductions larger than those obtained with faster freezing rates. It was found that complete thawing in freeze-thaw cycles was not necessary to achieve bactericidal action. The application of multiple freeze-thaw cycles in combination with low concentrations of isoeugenol could effectively reduce numbers of L. monocytogenes cells in bacteriological media.
Hayden K Cressy; Alistair R Jerrett; Carolyn M Osborne; Phil J Bremer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  66     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-14     Completed Date:  2003-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  390-5     Citation Subset:  IM    
New Zealand Institute for Crop and Food Research Limited, 300 Wakefield Quay, Nelson, New Zealand.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Colony Count, Microbial
Culture Media
Dose-Response Relationship, Drug
Eugenol / analogs & derivatives*,  pharmacology*
Food Microbiology
Listeria monocytogenes / drug effects,  growth & development*
Polysorbates / pharmacology*
Reg. No./Substance:
0/Culture Media; 0/Polysorbates; 97-53-0/Eugenol; 97-54-1/isoeugenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Viability of Clostridium perfringens, Escherichia coli, and Listeria monocytogenes surviving mild he...
Next Document:  Effects of acid adaptation and modified marinades on survival of postdrying Salmonella contamination...