Document Detail


A novel method for measuring dynamic changes in cell volume.
MedLine Citation:
PMID:  14594867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many cell types regulate their volume in response to extracellular tonicity changes through a complex series of adaptive mechanisms. Several methods that are presently used to measure cell volume changes include Coulter counters, fluorescent techniques, electronic impedance, and video microscopy. Although these methods are widely used and accepted, there are limitations associated with each technique. This paper describes a new method to measure changes in cell volume based on the principle that fluid flow within a rigid system is well determined. For this study, cos-7 cells were plated to line the inner lumen of a glass capillary and stimulated to swell or shrink by altering the osmolarity of the perfusing solution. The cell capillary was connected in series with a blank reference capillary, and differential pressure changes across each tube were monitored. The advantages of this method include 1) ability to continuously monitor changes in volume during rapid solution changes, 2) independence from cell morphology, 3) presence of physiological conditions with cell surface contacts and cell-cell interactions, 4) no phototoxic effects such as those associated with fluorescent methods, and 5) ability to report from large populations of cells. With this method, we could detect the previously demonstrated enhanced volume regulation of cells overexpressing the membrane phosphoprotein phospholemman, which has been implicated in osmolyte transport.
Authors:
Cristina E Davis; Joshua J Rychak; Bouvard Hosticka; Scott C Davis; J Edward John; Amy L Tucker; Pamela M Norris; J Randall Moorman
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-10-31
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  96     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-12     Completed Date:  2004-12-14     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1886-93     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
COS Cells
Calibration
Cell Size
Cercopithecus aethiops
Cytological Techniques*
Kidney / cytology*,  metabolism
Membrane Proteins / metabolism
Models, Biological
Phosphoproteins / metabolism
Transducers, Pressure
Grant Support
ID/Acronym/Agency:
5T32-HL-07284/HL/NHLBI NIH HHS; HL-70548/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Phosphoproteins; 135541-82-1/phospholemman

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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