Document Detail


A novel method for making "tissue" microarrays from small numbers of suspension cells.
MedLine Citation:
PMID:  15722798     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue microarrays (TMAs) are a highly efficient method for large-scale protein expression studies. To date most TMAs have been constructed using paraffin-embedded specimens. The authors developed a method that allows construction of TMAs from small numbers of cells in suspension. Spun pellets of 1x10 to 1x10 cells are directly processed and embedded in paraffin in an Eppendorf tube. Cylindrical cores of 0.6 mm are taken from these tubes and embedded in a recipient paraffin block to create a TMA. This relatively simple but versatile method enables very small numbers of cells in suspension to be analyzed using the TMA technology and allows for the study of hematolymphoid and related disorders of the blood and bone marrow for which solid tissue samples cannot be readily obtained. With the increasing trend toward obtaining small samples for screening and diagnostic purposes, this method provides a means to manipulate small volume samples for high-throughput immunohistochemical analysis. This method is also amenable for use for cultured cells.
Authors:
Kelli Montgomery; Shuchun Zhao; Matt van de Rijn; Yasodha Natkunam
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry     Volume:  13     ISSN:  1541-2016     ISO Abbreviation:  Appl. Immunohistochem. Mol. Morphol.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-21     Completed Date:  2005-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888796     Medline TA:  Appl Immunohistochem Mol Morphol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  80-4     Citation Subset:  IM    
Affiliation:
Department of Pathology, Stanford University Medical Center, Stanford, CA 94305, USA.
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MeSH Terms
Descriptor/Qualifier:
Bone Marrow Cells / pathology
Cell Culture Techniques / methods*
Hematologic Diseases / genetics,  pathology
Humans
Immunohistochemistry
Lymphatic Diseases / genetics,  pathology
Protein Array Analysis / methods*
Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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