Document Detail


A novel method for the determination of total 1,3-octanediols in apple juice via 1,3-dioxanes by solid-phase microextraction and high-speed gas chromatography.
MedLine Citation:
PMID:  20299018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this work, a novel, simple and fast method based on solid-phase microextraction (SPME) followed by high-speed gas chromatography (HSGC) was developed for the analysis of total 1,3-octanediols in apple juices by means of derivatization reaction to volatile 1,3-dioxanes. The derivatization reaction, SPME conditions, glycosidically bound fraction and 1,3-nonanediol as a surrogate standard were studied. The formation of 1,3-dioxanes from 1,3-diols was confirmed by GC-MS. The method was validated obtaining a regression coefficient (r(2)) of 0.9996, precisions between 0.3 and 9.8%, extraction recoveries in the range 94.7-112.2% and LOD of 2.9 microg l(-1). Experimental design has been employed in the optimization of extraction factors and robustness assessment. The method was applied to the analysis of 21 Asturian apple varieties finding a double reciprocal relationship between the concentrations of saturated and unsaturated 1,3-octanediol.
Authors:
Daniel D?az Llorente; Pilar Arias Abrodo; Enrique Dapena de la Fuente; Juan Jos? Mangas Alonso; Maria Dolores Guti?rrez Alvarez; Domingo Blanco Gomis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-03
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1217     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-12     Completed Date:  2010-06-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2993-9     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Affiliation:
Departamento de Qu?mica F?sica y Anal?tica, Universidad de Oviedo, C/ Juli?n Claver?a 8, E-33006 Oviedo, Spain.
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MeSH Terms
Descriptor/Qualifier:
Beverages / analysis*
Chromatography, Gas / methods*
Dioxanes / chemistry*
Malus / chemistry*
Molecular Structure
Octanes / analysis*,  chemistry
Solid Phase Microextraction / methods*
Chemical
Reg. No./Substance:
0/Dioxanes; 0/Octanes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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