Document Detail


A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes.
MedLine Citation:
PMID:  20521093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The aim of the present study was to investigate the effect of mouse oocyte volume on the efficiency of chromosomal analysis in livestock spermatozoa.
METHODS: Oocytes were injected with bull, ram, boar and dog sperm heads, and then fused with enucleated mouse oocytes.
RESULTS: The increment of oocyte volume increased the rates of morphologically normal oocytes after sperm injection, which induced much higher rates of overall chromosome detection in bull, ram and dog spermatozoa. The recipient oocyte volume did not affect the chromosomal integrity. Furthermore, in bull, the chromosomal integrity detected by fused mouse oocytes was similar to that derived from a homologous system. On the other hand, chromosomal plates of boar spermatozoa could not be detected despite the use of fused oocytes.
CONCLUSION: These data indicate that fused mouse oocytes improved the efficiency of chromosome detection in bull, ram and dog spermatozoa.
Authors:
Hiroyuki Watanabe; Hiroshi Suzuki; Hiroyuki Tateno; Yutaka Fukui
Related Documents :
9892723 - Production of androgenetic haploids in zebrafish with ultraviolet light.
8752623 - Birth of healthy children after preimplantation diagnosis of common aneuploidies by pol...
15905993 - A new aluminum tolerance gene located on rye chromosome arm 7rs.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-04
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  27     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-10-28     Completed Date:  2011-02-04     Revised Date:  2011-09-13    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  581-8     Citation Subset:  IM    
Affiliation:
Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, Japan. h-wata@asahikawa-med.ac.jp.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chromosome Aberrations*
Cryopreservation*
Dogs
Female
Logistic Models
Male
Mice
Microinjections
Oocytes*
Sheep
Sperm Injections, Intracytoplasmic
Spermatozoa / physiology*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Flavonoids, proanthocyanidins, and the risk of stomach cancer.
Next Document:  Multiplicity of molecular and cellular targets in human ovarian autoimmunity: an update.