Document Detail

A novel method for culturing sweat gland epithelia: comparison of normal and cystic fibrosis tissues.
MedLine Citation:
PMID:  2306416     Owner:  NLM     Status:  MEDLINE    
A new method is described to produce epithelial sheets by direct explantation of human sweat glands onto matrigel-coated millipore filters. The method is applicable to whole glands, separated coils or ducts and to normal and CF tissues. Electrogenic transport studies show that epithelia develop sodium transporting capability, even when explants are derived from secretory coils.
D J Brayden; A W Cuthbert
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of clinical pharmacology     Volume:  29     ISSN:  0306-5251     ISO Abbreviation:  Br J Clin Pharmacol     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-04-12     Completed Date:  1990-04-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7503323     Medline TA:  Br J Clin Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  235-8     Citation Subset:  IM    
Department of Pharmacology, University of Cambridge.
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MeSH Terms
Culture Techniques
Cystic Fibrosis / metabolism*
Epithelial Cells
Epithelium / metabolism
Sodium / metabolism
Sweat Glands / drug effects,  metabolism*
Reg. No./Substance:

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