Document Detail


A novel method of analysis of ligand-polynucleotide adsorption isotherms
MedLine Citation:
PMID:  11771272     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The adsorption of ethidium bromide on the synthetic double-stranded polyribonucleotide poly(G)poly(C) was investigated by adsorption spectroscopy. The adsorption isotherms were analyzed in the framework of the Crothers-Gursky model. It was shown that adsorption parameters (e.g., binding constant) may also be determined from the analysis of dispersion of some bound molecules. In some cases this method may be more convenient.
Authors:
Iu S Babaian; V B Arakelian; G G Potikian; R S Kazarian
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Biofizika     Volume:  46     ISSN:  0006-3029     ISO Abbreviation:  Biofizika     Publication Date:    2001 Nov-Dec
Date Detail:
Created Date:  2001-12-28     Completed Date:  2002-01-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372666     Medline TA:  Biofizika     Country:  Russia    
Other Details:
Languages:  rus     Pagination:  1003-5     Citation Subset:  IM    
Affiliation:
Yerevan State University, ul. A. Manukyan 1, Yerevan, 375049 Armenia.
Vernacular Title:
Novyĭ metod analiza izoterm adsorbtsii ligandov s polinukleotidami.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Algorithms
Ethidium / chemistry*
Ligands
Poly C / chemistry*
Poly G / chemistry*
Spectrophotometry
Chemical
Reg. No./Substance:
0/Ligands; 25191-14-4/Poly G; 25280-45-9/poly G-poly C; 30811-80-4/Poly C; 3546-21-2/Ethidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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