| A novel member of the BTB/POZ family, PATZ, associates with the RNF4 RING finger protein and acts as a transcriptional repressor. | |
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MedLine Citation:
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PMID: 10713105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have identified a novel human gene encoding a 59-kDa POZ-AT hook-zinc finger protein (PATZ) that interacts with RNF4, a mediator of androgen receptor activity, and acts as a transcriptional repressor. PATZ cDNA was isolated through a two-hybrid interaction screening using the RING finger protein RNF4 as a bait. In vitro and in vivo interaction between RNF4 and PATZ was demonstrated by protein-protein affinity chromatography and coimmunoprecipitation experiments. Such interaction occurred through a small region of PATZ containing an AT-hook DNA binding domain. Immunofluorescence staining and confocal microscopy showed that PATZ localizes in distinct punctate nuclear regions and colocalizes with RNF4. Functional analysis was performed by cotransfection assays: PATZ acted as a transcriptional repressor, whereas its partner RNF4 behaved as a transcriptional activator. When both proteins were overexpressed a strong repression of the basal transcription was observed, indicating that the association of PATZ with RNF4 switches activation to repression. In addition, RNF4 was also found to associate with HMGI(Y), a chromatin-modeling factor containing AT-hook domains. |
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Authors:
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M Fedele; G Benvenuto; R Pero; B Majello; S Battista; F Lembo; E Vollono; P M Day; M Santoro; L Lania; C B Bruni; A Fusco; L Chiariotti |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 275 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2000 Mar |
Date Detail:
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Created Date: 2000-04-12 Completed Date: 2000-04-12 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7894-901 Citation Subset: IM |
Affiliation:
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Centro di Endocrinologia ed Oncologia Sperimentale del CNR "G. Salvatore" Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano" Università degli Studi di Napoli "Federico II" via S. Pansini, 5, 80131 Napoli, Italy. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF119255; AF119256 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals DNA-Binding Proteins / metabolism HMGA1a Protein High Mobility Group Proteins / metabolism Humans Kruppel-Like Transcription Factors Mice Molecular Sequence Data Neoplasm Proteins* Nuclear Proteins* Protein Binding Repressor Proteins / classification, genetics, metabolism* Sequence Homology, Amino Acid Transcription Factors / metabolism Transcription, Genetic Two-Hybrid System Techniques Zinc Fingers* |
| Chemical | |
Reg. No./Substance:
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0/ABTB1 protein, human; 0/Abtb1 protein, mouse; 0/DNA-Binding Proteins; 0/High Mobility Group Proteins; 0/Kruppel-Like Transcription Factors; 0/Neoplasm Proteins; 0/Nuclear Proteins; 0/PATZ1 protein, human; 0/RNF4 protein, human; 0/Repressor Proteins; 0/Rnf4 protein, mouse; 0/Transcription Factors; 0/Zfp278 protein, mouse; 124544-67-8/HMGA1a Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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