| A novel mechanism for neutrophil priming in trauma: potential role of peritoneal fluid. | |
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MedLine Citation:
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PMID: 20466401 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We sought to determine the effect of peritoneal fluid from a novel animal model of abdominal compartment syndrome (ACS) on the proinflammatory status of polymorphonuclear leukocytes (PMNs) and monocytes. We hypothesize that peritoneal fluid is a potential priming and/or activating agent for PMNs/monocytes. METHODS: ACS was induced in female Yorkshire swine, and peritoneal fluid was collected at the time of decompressive laparotomy. Naïve PMNs/monocytes were primed and/or activated with peritoneal fluid, phosphatidylcholine (PAF) plus peritoneal fluid, peritoneal fluid plus n-formyl-met-leu-phe (fMLP), and peritoneal fluid plus phorbol 12-myristate 13-acetate (PMA). Activation was determined by surface marker expression of integrins (CD11b an CD18) and selectins (CD62L). Additionally, proinflammatory cytokines in peritoneal fluid were analyzed. RESULTS: Peritoneal fluid did not activate PMNs but increased CD11b expression on monocytes. When used as a primer for fMLP- or PMA-induced activation, peritoneal fluid significantly increased CD11b and CD18 expression on PMNs and monocytes. Peritoneal fluid collected at 6 and 12 h post decompressive laparotomy had similar effects. Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) levels were increased in peritoneal fluid. CONCLUSION: Peritoneal fluid represents a primer for PMNs/monocytes and seems to act through receptor-dependent and receptor-independent pathways. Strategies to reduce the amount of peritoneal fluid may decrease the locoregional and systemic inflammatory response by reducing priming and activation of neutrophils/monocytes. |
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Authors:
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Shinil K Shah; Fernando Jimenez; Peter A Walker; Kevin R Aroom; Hasen Xue; Teri D Feeley; Karen S Uray; Kenneth C Norbury; Randolph H Stewart; Glen A Laine; Charles S Cox |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-05-13 |
Journal Detail:
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Title: Surgery Volume: 148 ISSN: 1532-7361 ISO Abbreviation: Surgery Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-16 Completed Date: 2010-08-06 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0417347 Medline TA: Surgery Country: United States |
Other Details:
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Languages: eng Pagination: 263-70 Citation Subset: AIM; IM |
Copyright Information:
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Copyright 2010 Mosby, Inc. All rights reserved. |
Affiliation:
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Department of Pediatric Surgery, University of Texas Medical School at Houston, Houston, TX 77030, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascitic Fluid / physiology* Compartment Syndromes / etiology, physiopathology*, surgery Cytokines / physiology Decompression, Surgical Disease Models, Animal Female Inflammation Mediators / physiology Interleukin-6 / physiology Models, Biological Monocytes / physiology N-Formylmethionine Leucyl-Phenylalanine / pharmacology Neutrophil Activation / drug effects, physiology Neutrophils / drug effects, physiology* Resuscitation / adverse effects Sus scrofa Tumor Necrosis Factor-alpha / physiology Wounds and Injuries / complications, physiopathology* |
| Grant Support | |
ID/Acronym/Agency:
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K01 DK 070758/DK/NIDDK NIH HHS; K01 DK070758-04/DK/NIDDK NIH HHS; P50 GM 38529/GM/NIGMS NIH HHS; P50 GM038529-19/GM/NIGMS NIH HHS; R01 HL 36115/HL/NHLBI NIH HHS; R01 HL036115/HL/NHLBI NIH HHS; T32 GM 0879201/GM/NIGMS NIH HHS; T32 GM008792-09/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Inflammation Mediators; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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