| A novel mechanism of diglyceride formation. 12-O-tetradecanoylphorbol-13-acetate stimulates the cyclic breakdown and resynthesis of phosphatidylcholine. | |
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MedLine Citation:
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PMID: 3087989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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12-O-Tetradecanoylphorbol-13-acetate (TPA) treatment of Madin-Darby canine kidney cells resulted in an increased incorporation of 32Pi and [methyl-3H]choline into choline-containing phosphoglycerides (PC). In pulse-chase experiments, TPA treatment caused an increased release of [methyl-3H]choline from the PC fraction of prelabeled cells. When cells were prelabeled with [3H]arachidonic acid and [14C]palmitic acid, TPA treatment resulted in an increased synthesis of 14C, 3H-diglycerides. Further studies were done to determine the relationship between PC breakdown and diglyceride synthesis. Cells were preincubated with ether-linked 1-O-[3H]hexadecyl-2-lyso-sn-glycero-3-phosphocholine which was acylated to form 1-O-[3H]hexadecyl-2-acyl-sn-glycero-3-phosphocholine. Subsequent treatment of these cells with TPA resulted in an increased synthesis of 1-O-[3H]hexadecyl-2-acyl-sn-glycerol compared to cells not stimulated with TPA. These findings demonstrate that TPA stimulates PC turnover in Madin-Darby canine kidney cells and provide evidence for a novel mechanism of diglyceride formation. |
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Authors:
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L W Daniel; M Waite; R L Wykle |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 261 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1986 Jul |
Date Detail:
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Created Date: 1986-08-13 Completed Date: 1986-08-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9128-32 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid Arachidonic Acids / metabolism Cell Line Choline / metabolism Diglycerides / biosynthesis* Dogs Glycerides / biosynthesis* Kidney Palmitic Acid Palmitic Acids / metabolism Phorbols / pharmacology* Phosphates / metabolism Phosphatidylcholines / biosynthesis* Phosphatidylethanolamines / biosynthesis Platelet Activating Factor / analogs & derivatives, metabolism Tetradecanoylphorbol Acetate / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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AI-17287/AI/NIAID NIH HHS; HL-26818/HL/NHLBI NIH HHS; HL-31338/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Diglycerides; 0/Glycerides; 0/Palmitic Acids; 0/Phorbols; 0/Phosphates; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Platelet Activating Factor; 16561-29-8/Tetradecanoylphorbol Acetate; 506-32-1/Arachidonic Acid; 57-10-3/Palmitic Acid; 62-49-7/Choline; 74389-68-7/1-hexadecyl-2-acetyl-glycero-3-phosphocholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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