| A novel lipopeptide, an inhibitor of bacterial adhesion, from the thermophilic and halotolerant subsurface Bacillus licheniformis strain 603. | |
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MedLine Citation:
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PMID: 14643798 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new Bacillus licheniformis strain, 603, isolated from a mixture of drilling fluid and subsurface thermal water, has been found to produce a cyclic lipopeptide which is released into cultural medium as well as present in cells as the major lipid constituent (57% of the total cell lipids extractable with 2:1 chloroform-methanol). The quantitative ratio of the extracellular and intracellular lipopeptide has been estimated as 23:10. The metabolite represents a heptapeptide, L-Asp-->L-Leu-->L-Leu-->L-Val-->L-Val-->L-Glu-->L-Leu, N-acylated to the N-terminal amino acid, L-Asp, by a 3-hydroxy fatty acid (from 13:0 to 17:0 with n-, iso-, and anteiso-chains), the 3-OH group of which is esterified by the C-terminal amino acid, L-Leu. The chemical structure of the lipopeptide has been established by means of infrared (IR), 1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy, electrospray ionisation (ESI) mass spectrometry (MS), including secondary ion mass spectrometry, along with chemical and enzymatic degradation. Although a diversity of similar metabolites synthesised by various B. licheniformis strains are presently known, such a structure has not been reported thus far. Added to the growth medium of strain 603 at the concentration of 1.6 microg/ml, the lipopeptide prevents adhesion of cells to a glass surface. Also, it exhibits a considerable growth-inhibiting activity against Corynebacterium variabilis and a much lower activity against Acinetobacter sp. |
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Authors:
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Stanislav G Batrakov; Tatiana A Rodionova; Stanislav E Esipov; Nikita B Polyakov; Vladimir I Sheichenko; Nina V Shekhovtsova; Sergey M Lukin; Nikolai S Panikov; Yuri A Nikolaev |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1634 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-12-03 Completed Date: 2004-01-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 107-15 Citation Subset: IM |
Affiliation:
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Russian Research Centre Hydrobios of Ministry of Health, ul. Kosmonavtov 18, korp. 2, Moscow 129301, Russia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acinetobacter
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drug effects,
growth & development Amino Acid Sequence Bacillus / chemistry*, isolation & purification Bacterial Adhesion / drug effects* Corynebacterium / drug effects, growth & development Lipoproteins* / chemistry, isolation & purification, pharmacology Molecular Structure Peptides, Cyclic / chemistry, isolation & purification, pharmacology Soil Microbiology* Spectrum Analysis |
| Chemical | |
Reg. No./Substance:
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0/Lipoproteins; 0/Peptides, Cyclic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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