Document Detail

A novel lipopeptide, an inhibitor of bacterial adhesion, from the thermophilic and halotolerant subsurface Bacillus licheniformis strain 603.
MedLine Citation:
PMID:  14643798     Owner:  NLM     Status:  MEDLINE    
A new Bacillus licheniformis strain, 603, isolated from a mixture of drilling fluid and subsurface thermal water, has been found to produce a cyclic lipopeptide which is released into cultural medium as well as present in cells as the major lipid constituent (57% of the total cell lipids extractable with 2:1 chloroform-methanol). The quantitative ratio of the extracellular and intracellular lipopeptide has been estimated as 23:10. The metabolite represents a heptapeptide, L-Asp-->L-Leu-->L-Leu-->L-Val-->L-Val-->L-Glu-->L-Leu, N-acylated to the N-terminal amino acid, L-Asp, by a 3-hydroxy fatty acid (from 13:0 to 17:0 with n-, iso-, and anteiso-chains), the 3-OH group of which is esterified by the C-terminal amino acid, L-Leu. The chemical structure of the lipopeptide has been established by means of infrared (IR), 1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy, electrospray ionisation (ESI) mass spectrometry (MS), including secondary ion mass spectrometry, along with chemical and enzymatic degradation. Although a diversity of similar metabolites synthesised by various B. licheniformis strains are presently known, such a structure has not been reported thus far. Added to the growth medium of strain 603 at the concentration of 1.6 microg/ml, the lipopeptide prevents adhesion of cells to a glass surface. Also, it exhibits a considerable growth-inhibiting activity against Corynebacterium variabilis and a much lower activity against Acinetobacter sp.
Stanislav G Batrakov; Tatiana A Rodionova; Stanislav E Esipov; Nikita B Polyakov; Vladimir I Sheichenko; Nina V Shekhovtsova; Sergey M Lukin; Nikolai S Panikov; Yuri A Nikolaev
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1634     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-12-03     Completed Date:  2004-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  107-15     Citation Subset:  IM    
Russian Research Centre Hydrobios of Ministry of Health, ul. Kosmonavtov 18, korp. 2, Moscow 129301, Russia.
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MeSH Terms
Acinetobacter / drug effects,  growth & development
Amino Acid Sequence
Bacillus / chemistry*,  isolation & purification
Bacterial Adhesion / drug effects*
Corynebacterium / drug effects,  growth & development
Lipoproteins* / chemistry,  isolation & purification,  pharmacology
Molecular Structure
Peptides, Cyclic / chemistry,  isolation & purification,  pharmacology
Soil Microbiology*
Spectrum Analysis
Reg. No./Substance:
0/Lipoproteins; 0/Peptides, Cyclic

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