Document Detail


A novel label-free electrochemical microRNA biosensor using Pd nanoparticles as enhancer and linker.
MedLine Citation:
PMID:  23291596     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In this paper, a novel, label-free amperometric biosensor for the detection of microRNA-155 based on the conductive self-assembled multilayer comprised of Nafion, thionine (Thi) and Pd nanoparticles was successfully prepared. Nafion was firstly dropped on to a bare glass carbon electrode. Then Thi was absorbed by the cation exchanger Nafion. Furthermore, a Pd nanoparticles layer which was used to immobilize target biomolecules was constructed by the amino group of Thi as linker. Moreover, the proposed biosensor showed excellent electrocatalytic activity towards H(2)O(2) and enhanced the current response of the biosensor. Cyclic voltammetry was used for the characterization and electrochemical properties of the stepwise self-assembly process of the biosensor, and scanning electron microscopy was used for observing the microstructure of the modified film. Using microRNA-155 as a model, the resulting biosensor presented high sensitivity, good stability and a broad linear response from 5.6 to 5.6 × 10(5) pM with the detection limit of 1.87 pM under optimization of the assay conditions.
Authors:
Xiaoyan Wu; Yaqin Chai; Ruo Yuan; Huilan Su; Jing Han
Related Documents :
15028496 - Preparation and characterization of silver/tio2 composite hollow spheres.
18351326 - Size characterization of drug-loaded polymeric core/shell nanoparticles using asymmetri...
19441546 - Preparation and photocatalytic property of tio2-fe3o4 core-shell nanoparticles.
18817426 - Microwave, photo- and thermally responsive pnipam-gold nanoparticle microgels.
9466856 - The effect of many-body interactions on the sedimentation of monodisperse particle disp...
22181416 - Friction contribution to water-bond breakage kinetics.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-7
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. yuanruo@swu.edu.cn.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Preparation, preliminary screening of new types of steroid conjugates and their activities on steroi...
Next Document:  MHC class I-restricted myelin epitopes are cross-presented by Tip-DCs that promote determinant sprea...