Document Detail

A novel label-free electrochemical microRNA biosensor using Pd nanoparticles as enhancer and linker.
MedLine Citation:
PMID:  23291596     Owner:  NLM     Status:  Publisher    
In this paper, a novel, label-free amperometric biosensor for the detection of microRNA-155 based on the conductive self-assembled multilayer comprised of Nafion, thionine (Thi) and Pd nanoparticles was successfully prepared. Nafion was firstly dropped on to a bare glass carbon electrode. Then Thi was absorbed by the cation exchanger Nafion. Furthermore, a Pd nanoparticles layer which was used to immobilize target biomolecules was constructed by the amino group of Thi as linker. Moreover, the proposed biosensor showed excellent electrocatalytic activity towards H(2)O(2) and enhanced the current response of the biosensor. Cyclic voltammetry was used for the characterization and electrochemical properties of the stepwise self-assembly process of the biosensor, and scanning electron microscopy was used for observing the microstructure of the modified film. Using microRNA-155 as a model, the resulting biosensor presented high sensitivity, good stability and a broad linear response from 5.6 to 5.6 × 10(5) pM with the detection limit of 1.87 pM under optimization of the assay conditions.
Xiaoyan Wu; Yaqin Chai; Ruo Yuan; Huilan Su; Jing Han
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-7
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
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