Document Detail


A novel Fe(II)/α-ketoglutarate-dependent dioxygenase from Burkholderia ambifaria has β-hydroxylating activity of N-succinyl L-leucine.
MedLine Citation:
PMID:  22967283     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An Fe(II)/α-ketoglutarate-dependent dioxygenase, SadA, was obtained from Burkholderia ambifaria AMMD and heterologously expressed in Escherichia coli. Purified recombinant SadA had catalytic activity toward several N-substituted L-amino acids, which was especially strong with N-succinyl L-leucine. With the NMR and LC-MS analysis, SadA converted N-succinyl L-leucine into N-succinyl L-threo-β-hydroxyleucine with >99% diastreoselectivity. SadA is the first enzyme catalyzing β-hydroxylation of aliphatic amino acid-related substances and a potent biocatalyst for preparation of optically active β-hydroxy amino acids. © 2012 The Authors Letters in Applied Microbiology © 2012 The Society for AppliedMicrobiology.
Authors:
M Hibi; T Kawashima; T Kasahara; P M Sokolov; S V Smirnov; T Kodera; M Sugiyama; S Shimizu; K Yokozeki; J Ogawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-12
Journal Detail:
Title:  Letters in applied microbiology     Volume:  -     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for AppliedMicrobiology.
Affiliation:
Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
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