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A novel in vivo rabbit model of abdominal aortic aneurysm induced by periarterial incubation of papain.
MedLine Citation:
PMID:  23101926     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
PURPOSE: The objective of this study was to determine the possibility of creating a novel animal model of abdominal aortic aneurysm (AAA) in rabbits by the periarterial application of papain.
MATERIALS AND METHODS: Twelve New Zealand white rabbits were randomized into two groups: (1) the papain group, which received 2mg of papain (n=8) and (2) the control group, which received physiologic saline solution (n=4). A 1-cm aortic segment proximal to the bifurcation was isolated, and its adventitia was incubated with papain for 20 minutes. The rabbits underwent intravenous digital subtraction angiography (IVDSA) 5 and 21 days after the operation. The animals were then humanely killed for histomorphometric and immunohistochemical studies.
RESULTS: All animals in the papain group developed AAA, with an average aneurysm diameter of 4.0±0.6 and 4.1±0.4mm on days 5 and 21, respectively. No aneurysms were seen in the control group. On day 5, the papain-incubated aortas exhibited thinned and disorganized aortic walls, with decreased smooth muscle cells (SMCs) and fragmented and almost nonexistent elastic lamella. Media thickening, intimal hyperplasia, and smooth muscle cell regeneration were obvious on day 21. Immunostaining of matrix metalloproteinase (MMP)-9 and RAM11 showed strong expression in the papain group. On the contrary, the control group did not present histologic alterations and showed almost no expression of MMP-9 and RAM11.
CONCLUSIONS: A novel in vivo rabbit model of AAA can be induced through periarterial application of papain for 20 minutes. This model is similar to an elastase-induced aneurysm model and could be useful to clarify AAA pathogenesis and endovascular treatment intervention.
Authors:
Yonghua Bi; Ke Xu; Hongshan Zhong; Xun Qi; Zhen Zhang; Yicheng Ni
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of vascular and interventional radiology : JVIR     Volume:  23     ISSN:  1535-7732     ISO Abbreviation:  J Vasc Interv Radiol     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9203369     Medline TA:  J Vasc Interv Radiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1529-36     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 SIR. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Radiology (Y.B., K.X., H.Z., X.Q., Z.Z., Y.N), Laboratory of Medical Imaging and Interventional Radiology, the First Affiliated Hospital of China Medical University, Shenyang, China; Department of Ultrasound (Z.Z.), Affiliated First Hospital, China Medical University, Shenyang, China; and Department of Radiology (Y.N.), University Hospital, KU Leuven, Leuven, Belgium.
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