| A novel in vitro method for investigating cartilage degradation. | |
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MedLine Citation:
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PMID: 1365467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel in vitro microassay is described which allows the direct effects of short-lived cells or transient cell processes on cartilage matrix to be investigated. The method involves layering cells onto 2 microns cryostat sections of cartilage and assessing matrix integrity with quantitative histochemistry. Rat polymorphonuclear neutrophils (PMNs) caused glycosaminoglycan (GAG) loss from sections of bovine nasal cartilage. This loss of GAG was greatly enhanced by the presence of zymosan, phorbol ester or calcium ionophore. Similar results were obtained using human articular cartilage with human PMNs. This technique may be useful in the detection of novel chondroprotective agents. |
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Authors:
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A R Moore; M el-Ghazaly; D A Willoughby |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of rheumatology and inflammation Volume: 11 ISSN: 0140-1610 ISO Abbreviation: Eur J Rheumatol Inflamm Publication Date: 1991 |
Date Detail:
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Created Date: 1995-02-02 Completed Date: 1995-02-02 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7805765 Medline TA: Eur J Rheumatol Inflamm Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 13-8 Citation Subset: IM |
Affiliation:
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Department of Experimental Pathology, St. Bartholomew's Hospital Medical College, London. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcimycin / pharmacology Cartilage, Articular / chemistry, pathology* Cattle Cells, Cultured Densitometry Glycosaminoglycans / analysis* Humans Male Nasal Septum / chemistry, pathology* Neutrophils / physiology* Rats Rats, Wistar Tetradecanoylphorbol Acetate / pharmacology Zymosan / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Glycosaminoglycans; 16561-29-8/Tetradecanoylphorbol Acetate; 52665-69-7/Calcimycin; 9010-72-4/Zymosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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