Document Detail


A novel in vitro assessment of tissue valve calcification by a continuous flow type method.
MedLine Citation:
PMID:  10718770     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A dynamic flow type testing to study calcification was self-designed to investigate calcification in bioprosthetic heart valves. The apparatus consists of a container into which leaflets from a porcine aortic valve are placed, a chamber that contains calcium solution, and a peristaltic pump that provides a continuous supply of the solution toward the container. Efficacy of the apparatus was compared with the conventional batch type calcification testing at 37 degrees C through measuring the amount of calcium and phosphate deposited by inductively coupled plasma (ICP) and scanning electron microscope (SEM). After 14 days, calcium levels detected from the calcified deposit on leaflets were 470.4 +/- 37.0 microg/cm3 in the flow type testing whereas in the batch type testing levels were 81.0 +/- 6.7 microg/cm3. Though the calcium level on the leaflet increased as the exposure time to calcium solution increased in both testings, the rate and the tendency of calcification could be assessed very rapidly by flow type testing in comparison with batch type testing. [Ca]/[P] molar ratio decreased over time, and after 14 days, the ratio was close to 1.83 +/- 0.18 in the flow type testing. The ratio could not be determined in the batch type testing because the deposit was too small to assess. The descending rate of [Ca]/[P] molar ratio demonstrates that deposited calcium-complex at the earliest stage may interact with inorganic phosphate ions to create a calcified deposit mineral precursor. This in vitro dynamic flow type calcification testing was a favorable tool for rapid investigation of calcification.
Authors:
J C Park; Y S Hwang; D W Han; H Suh
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artificial organs     Volume:  24     ISSN:  0160-564X     ISO Abbreviation:  Artif Organs     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-04-04     Completed Date:  2000-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  158-60     Citation Subset:  IM    
Affiliation:
Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Aortic Valve* / chemistry,  ultrastructure
Bioprosthesis*
Calcinosis / diagnosis*
Calcium / analysis
Heart Valve Prosthesis*
Humans
Microscopy, Electron, Scanning
Phosphates / analysis
Rheology
Chemical
Reg. No./Substance:
0/Phosphates; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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