Document Detail


A novel host responsive sensor mediates virulence and type III secretion during Pseudomonas aeruginosa-host cell interactions.
MedLine Citation:
PMID:  22262100     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Sensitive sensory mechanisms are instrumental in affording Pseudomonas aeruginosa the capacity to establish diverse yet severe human infections, which can manifest in long term untreatable disease. The ability of P. aeruginosa to tightly regulate gene expression and virulence factor production, in response to activation of these sensory components, enables the pathogen to sustain infection despite the host immune response and aggressive antibiotic treatment. Although a number of factors are recognised as playing a role in early infection, very little is known regarding the sensors involved in this process. In this study, we identified P. aeruginosa PA3191 as a novel host responsive sensor that played a key role during P. aeruginosa-host interactions and was required for optimum colonisation and dissemination in a mouse model of infection. We demonstrated PA3191 contributed to modulation of the Type III Secretion System (T3SS) in response to host cells and T3SS inducing conditions in vitro. PA3191 (designated GtrS) acted in concert with the response regulator GltR to regulate the OprB transport system and subsequently carbon metabolism. Through this signal transduction pathway, T3SS activation was mediated via the RsmAYZ regulatory cascade and involved the global anaerobic response regulator Anr.
Authors:
Julie O'Callaghan; Jerry F Reen; Claire Adams; Pat G Casey; Cormac G Gahan; Fergal O'Gara
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-19
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University College Cork.
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