| A novel histone deacetylase inhibitor, CG0006, induces cell death through both extrinsic and intrinsic apoptotic pathways. | |
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MedLine Citation:
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PMID: 19644355 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Histone deacetylase inhibitors (HDACIs) are potent anticancer drugs, and suberoylanilide hydroxamic acid is used for the treatment of cutaneous T-cell lymphoma patients. We synthesized a novel hydroxamate-based HDACI, CG0006, and assessed its antiproliferative effects on the NCI-60 cancer cell panel and cell lines from liver and stomach cancers that are common in Korea. Micromolar levels of CG0006 induced cell death in several breast, central nervous system, colon, hematopoietic, lung, melanoma, ovarian, prostatic, renal, and stomach cancer cell lines. We further analyzed cell death mechanisms activated by CG0006 in HCT116 (colon cancer) and K562 (leukemia) cells. First, to test the activity of CG0006, we analyzed acetylation of substrates of HDACs and effect on gene expression. CG0006 increased acetylation of histone 3, histone 4, and tubulin in a time-dependent and dose-dependent manner in both HCT116 and K562 cells. Moreover, CG0006 increased the mRNA level of p21 and decreased that of Bcl-xl efficiently in HCT116 cells. Cell cycle analysis showed G2-M arrest, and increased apoptosis in populations of HCT116 and K562 cells treated with CG0006. Western blot analysis showed that CG0006 increased levels of p21 in HCT116 cells and of p21 and p27 in K562 cells. In addition, CG0006 activated caspase-9, caspase-3, and caspase-8. These results indicate that CG0006 induces death in HCT116 and K562 cells through both intrinsic and extrinsic apoptotic pathways. The HDACI CG0006 may be a potent anticancer drug for solid tumors and leukemia. |
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Authors:
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Jung Jin Hwang; Yong Sook Kim; Mi Joung Kim; Sejin Jang; Je-Hwan Lee; Jene Choi; Seonggu Ro; Young-Lan Hyun; Jung Shin Lee; Choung-Soo Kim |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anti-cancer drugs Volume: 20 ISSN: 1473-5741 ISO Abbreviation: Anticancer Drugs Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-03 Completed Date: 2009-12-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9100823 Medline TA: Anticancer Drugs Country: England |
Other Details:
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Languages: eng Pagination: 815-21 Citation Subset: IM |
Affiliation:
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Institute for Innovative Cancer Research, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation
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drug effects Caspases / metabolism Cell Cycle / drug effects Cell Death / drug effects* Cell Line, Tumor Cyclin-Dependent Kinase Inhibitor p21 / genetics, metabolism Cyclin-Dependent Kinase Inhibitor p27 / metabolism Enzyme Inhibitors / chemistry, pharmacology* Gene Expression / drug effects Gene Expression Regulation, Neoplastic / drug effects Histone Deacetylase Inhibitors / metabolism* Histones / metabolism Humans Hydroxamic Acids / chemical synthesis, chemistry, pharmacology* Piperidines / chemical synthesis, pharmacology* Tubulin / metabolism bcl-X Protein / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/CG 0006; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Enzyme Inhibitors; 0/Histone Deacetylase Inhibitors; 0/Histones; 0/Hydroxamic Acids; 0/PXD101; 0/Piperidines; 0/Tubulin; 0/bcl-X Protein; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 149647-78-9/vorinostat; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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