Document Detail


A novel high resolution in vivo digital imaging system for the evaluation of experimental cataract in diabetic rats.
MedLine Citation:
PMID:  18187926     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to establish a novel imaging system for evaluation of cataract in small animals. Diabetic cataract was induced in male Wistar rats by a combination of a bolus injection of streptozotocin (65 mg/kg, i.v.) and 5% D-glucose given as drinking water. To assess cataract development, we designed a digital camera system equipped with a non-reflecting illuminator for capturing clear high-resolution full lens images in the horizontal plane. Cataract was evaluated from the resulting images using both an observer-based scoring system and quantitative digital image-analysis techniques. The onset of cataract was detected in the peripheral section of the lens in 57% of cases 2 weeks after induction of hyperglycemia. Central opacities were visible following 3 weeks in hyperglycemic conditions. The cataract increased in severity with time, so that by week 9 in hyperglycemic conditions, mature cataracts were developed in over 90% of lenses. Treatment of diabetic rats with GP-1447, an aldose reductase inhibitor, completely prevented the formation of diabetic cataracts. These results indicate that the digital imaging system established in the present study permits an assessment of all stages of cataract development and it is helpful for accurately evaluating the effects of therapeutic drugs on cataracts.
Authors:
Sokichi Kametaka; Takafumi Kasahara; Mayumi Ueo; Mariko Takenaka; Maki Saito; Kenji Sakamoto; Tsutomu Nakahara; Kunio Ishii
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2008-01-11
Journal Detail:
Title:  Journal of pharmacological sciences     Volume:  106     ISSN:  1347-8613     ISO Abbreviation:  J. Pharmacol. Sci.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-23     Completed Date:  2008-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101167001     Medline TA:  J Pharmacol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  144-51     Citation Subset:  IM    
Affiliation:
Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences, Minato-ku, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aldehyde Reductase / antagonists & inhibitors,  metabolism
Animals
Blood Glucose / metabolism
Body Weight
Cataract / enzymology,  etiology,  pathology*,  prevention & control
Diabetes Mellitus, Experimental / complications*,  drug therapy,  enzymology,  pathology
Drinking
Eating
Enzyme Inhibitors / pharmacology,  therapeutic use
Equipment Design
Image Interpretation, Computer-Assisted*
Lens, Crystalline / drug effects,  enzymology,  pathology*
Male
Phenylacetates / pharmacology,  therapeutic use
Rats
Rats, Wistar
Reproducibility of Results
Severity of Illness Index
Signal Processing, Computer-Assisted / instrumentation*
Thiazoles / pharmacology,  therapeutic use
Time Factors
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Enzyme Inhibitors; 0/GP 1447; 0/Phenylacetates; 0/Thiazoles; EC 1.1.1.21/Aldehyde Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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