Document Detail

A novel glutamate dehydrogenase from bovine brain: purification and characterization.
MedLine Citation:
PMID:  7581004     Owner:  NLM     Status:  MEDLINE    
A soluble form of novel glutamate dehydrogenase has been purified from bovine brain. The preparation was homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and composed of six identical subunits having a subunit size of 57,500 Da. The biochemical properties of glutamate dehydrogenase such as N-terminal amino acids sequences, kinetic parameters, amino acids analysis, and optimum pH were examined in both reductive amination of alpha-ketoglutarate and oxidative deamination of glutamate. N-terminal amino acid sequences of the bovine brain enzyme showed the significant differences in the first 5 amino acids compared to other glutamate dehydrogenases from various sources. These results indicate that glutamate dehydrogenase isolated from bovine brain is a novel polypeptide.
J Lee; S W Kim; S W Cho
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry and molecular biology international     Volume:  36     ISSN:  1039-9712     ISO Abbreviation:  Biochem. Mol. Biol. Int.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-12-22     Completed Date:  1995-12-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9306673     Medline TA:  Biochem Mol Biol Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  1087-96     Citation Subset:  IM    
Department of Biochemistry, College of Medicine, University of Ulsan, Seoul, Korea.
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MeSH Terms
Amino Acid Sequence
Amino Acids / analysis
Brain / enzymology*
Coenzymes / metabolism
Glutamate Dehydrogenase / chemistry*,  isolation & purification*,  metabolism
Glutamic Acid
Hydrogen-Ion Concentration
Ketoglutaric Acids / metabolism
Molecular Sequence Data
Molecular Weight
Sequence Analysis
Substrate Specificity
Reg. No./Substance:
0/Amino Acids; 0/Coenzymes; 0/Ketoglutaric Acids; 328-50-7/alpha-ketoglutaric acid; 56-86-0/Glutamic Acid; EC Dehydrogenase

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