| A novel glutamate dehydrogenase from bovine brain: purification and characterization. | |
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MedLine Citation:
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PMID: 7581004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A soluble form of novel glutamate dehydrogenase has been purified from bovine brain. The preparation was homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and composed of six identical subunits having a subunit size of 57,500 Da. The biochemical properties of glutamate dehydrogenase such as N-terminal amino acids sequences, kinetic parameters, amino acids analysis, and optimum pH were examined in both reductive amination of alpha-ketoglutarate and oxidative deamination of glutamate. N-terminal amino acid sequences of the bovine brain enzyme showed the significant differences in the first 5 amino acids compared to other glutamate dehydrogenases from various sources. These results indicate that glutamate dehydrogenase isolated from bovine brain is a novel polypeptide. |
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Authors:
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J Lee; S W Kim; S W Cho |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry and molecular biology international Volume: 36 ISSN: 1039-9712 ISO Abbreviation: Biochem. Mol. Biol. Int. Publication Date: 1995 Aug |
Date Detail:
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Created Date: 1995-12-22 Completed Date: 1995-12-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9306673 Medline TA: Biochem Mol Biol Int Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 1087-96 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, College of Medicine, University of Ulsan, Seoul, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / analysis Animals Brain / enzymology* Cattle Coenzymes / metabolism Glutamate Dehydrogenase / chemistry*, isolation & purification*, metabolism Glutamic Acid Hydrogen-Ion Concentration Ketoglutaric Acids / metabolism Kinetics Molecular Sequence Data Molecular Weight Oxidation-Reduction Sequence Analysis Solubility Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Coenzymes; 0/Ketoglutaric Acids; 328-50-7/alpha-ketoglutaric acid; 56-86-0/Glutamic Acid; EC 1.4.1.2/Glutamate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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