| A novel gelatin hydrogel carrier sheet for corneal endothelial transplantation. | |
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MedLine Citation:
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PMID: 21534849 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We examined the feasibility of using gelatin hydrogels as carrier sheets for the transplantation of cultivated corneal endothelial cells. The mechanical properties, transparency, and permeability of gelatin hydrogel sheets were compared with those of atelocollagen sheets. Immunohistochemistry (ZO-1, Na+/K+-ATPase and N-cadherin), hematoxylin and eosin (HE) staining, and scanning electron microscopy (SEM) were performed to assess the integrity of corneal endothelial cells that were cultured on gelatin hydrogel sheets. The gelatin hydrogel sheets displayed greater transparency, elastic modulus, and albumin permeability compared to those of atelocollagen sheets. The corneal endothelial cells on gelatin hydrogel sheets showed normal expression levels of ZO-1, Na+/K+-ATPase and N-cadherin. HE staining revealed the formation of a continuous monolayer of cells attached to the gelatin hydrogel sheet. SEM observations showed that the corneal endothelial cells were arranged in a regular, mosaic and polygonal pattern with normal cilia. These results indicate that the gelatin hydrogel sheet is a promising material to transport corneal endothelial cells during transplantation. |
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Authors:
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Ryou Watanabe; Ryuhei Hayashi; Yu Kimura; Yuji Tanaka; Tomofumi Kageyama; Susumu Hara; Yasuhiko Tabata; Kohji Nishida |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-3 |
Journal Detail:
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Title: Tissue engineering. Part A Volume: - ISSN: 1937-335X ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Tohoku University Graduate School of Medicine, Department of Ophthalmology, Sendai, Japan; ryo-w@oph.med.tohoku.ac.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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