Document Detail


A novel function for the competence inducing peptide, XIP, as a cell death effector of Streptococcus mutans.
MedLine Citation:
PMID:  22900705     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Streptococcus mutans, ComX, an alternative sigma factor, drives the transcription of the 'late-competence genes' required for genetic transformation. ComX activity is modulated by inputs from two signaling pathways, ComDE and ComRS, that respond to the competence-stimulating peptide (CSP) and the SigX-inducing peptide (XIP), respectively. In particular, the comRS, encoding the ComR regulatory protein and the ComS precursor to XIP, functions as the proximal regulatory system for ComX activation. Here, we investigated the individual and combinatorial effects of CSP and XIP on genetic transformation and cell killing of S. mutans. Our transformation results confirm the recent reports by Mashburn-Warren et al. and Desai et al. that XIP functions optimally in a chemically defined medium, whereas its activity is inhibited when cells are grown in complex medium. Using tandem mass spectrometry (MS/MS) fragmentation, a drastic reduction in XIP levels in ComX-deficient cultures were observed, suggesting a ComX-mediated positive feedback mechanism for XIP synthesis. Our evaluation of cell viability in the presence of 10 μM XIP resulted in killing nearly 82% of the population. The killing activity was shown to be dependent on the presence of comR/S and comX. These results suggest a novel role for XIP as a compelling effector of cell death. This is the first report that demonstrates a role for XIP in cell killing.
Authors:
Iwona B Wenderska; Nikola Lukenda; Martha Cordova; Nathan Magarvey; Dennis G Cvitkovitch; Dilani B Senadheera
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-09-26
Journal Detail:
Title:  FEMS microbiology letters     Volume:  336     ISSN:  1574-6968     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-22     Completed Date:  2013-03-14     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  104-12     Citation Subset:  IM    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Department of Oral Microbiology, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Base Sequence
Culture Media
Gene Expression Regulation, Bacterial
Gene Order
Models, Biological
Mutation
Peptides / metabolism*
Signal Transduction
Streptococcus mutans / genetics,  growth & development,  metabolism*
Transcription Factors / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
MT15431//Canadian Institutes of Health Research; R01 DE013230/DE/NIDCR NIH HHS; R01DE013230-03/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/ComX protein, Streptococcus; 0/Culture Media; 0/Peptides; 0/Transcription Factors

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