Document Detail


A novel function of bamboo extract in relieving lipotoxicity.
MedLine Citation:
PMID:  18350521     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipotoxicity is closely related to the etiology and complications of type 2 diabetes mellitus. This study investigated the protective effect of an extract from bamboo Phyllostachys edulis against palmitic acid (PA)-induced lipoapoptosis. The lipo-detoxification function of the bamboo extract (BEX) was evaluated using cell culture models. Cell viability was measured by MTT assay and cell apoptosis was monitored by Annexin V staining. Cellular uptake of fluorescent free fatty acid (FFA) analog was measured by flow cytometry. Protein levels of total protein kinase B (Akt) and phosphorylated Akt (p-Akt) were measured by western blotting. The results show that co-incubating BEX with mouse myoblast C2C12 cells had no effect on the cellular uptake of FFA, but dramatically decreased PA-induced cell apoptosis and protected cell viability. A similar antilipotoxicity effect of BEX was observed in other mammalian cells. BEX significantly decreased the protein levels of both Akt and p-Akt in C2C12 cells under normal cell culture conditions but not under lipotoxic conditions, indicating the regulatory effect of BEX on cell signaling pathways and its response to a high FFA environment. This study demonstrated a novel function of bamboo extract in preventing lipotoxicity in mammalian cells, implicating a promising phytotherapeutic approach for lipo-detoxification.
Authors:
Jun Panee; Wanyu Liu; Yanling Lin; Christy Gilman; Marla J Berry
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  22     ISSN:  1099-1573     ISO Abbreviation:  Phytother Res     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-05     Completed Date:  2008-07-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  675-80     Citation Subset:  IM    
Affiliation:
Department of Cell and Molecular Biology, John A Burns School of Medicine, University of Hawaii at Manoa, 651 Ilalo Street BSB 222, Honolulu, HI 96813, USA. junchen@hawaii.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Bambusa / chemistry*
Blotting, Western
Cell Line
Cell Survival / drug effects
Cercopithecus aethiops
Fatty Acids, Nonesterified / metabolism
Flow Cytometry
Mice
Palmitic Acid / toxicity*
Plant Extracts / chemistry,  pharmacology*
Proto-Oncogene Proteins c-akt / metabolism
Vero Cells
Grant Support
ID/Acronym/Agency:
R21 AT003874-01/AT/NCCAM NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Plant Extracts; 57-10-3/Palmitic Acid; EC 2.7.11.1/Proto-Oncogene Proteins c-akt

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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