| A novel function of bamboo extract in relieving lipotoxicity. | |
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MedLine Citation:
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PMID: 18350521 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipotoxicity is closely related to the etiology and complications of type 2 diabetes mellitus. This study investigated the protective effect of an extract from bamboo Phyllostachys edulis against palmitic acid (PA)-induced lipoapoptosis. The lipo-detoxification function of the bamboo extract (BEX) was evaluated using cell culture models. Cell viability was measured by MTT assay and cell apoptosis was monitored by Annexin V staining. Cellular uptake of fluorescent free fatty acid (FFA) analog was measured by flow cytometry. Protein levels of total protein kinase B (Akt) and phosphorylated Akt (p-Akt) were measured by western blotting. The results show that co-incubating BEX with mouse myoblast C2C12 cells had no effect on the cellular uptake of FFA, but dramatically decreased PA-induced cell apoptosis and protected cell viability. A similar antilipotoxicity effect of BEX was observed in other mammalian cells. BEX significantly decreased the protein levels of both Akt and p-Akt in C2C12 cells under normal cell culture conditions but not under lipotoxic conditions, indicating the regulatory effect of BEX on cell signaling pathways and its response to a high FFA environment. This study demonstrated a novel function of bamboo extract in preventing lipotoxicity in mammalian cells, implicating a promising phytotherapeutic approach for lipo-detoxification. |
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Authors:
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Jun Panee; Wanyu Liu; Yanling Lin; Christy Gilman; Marla J Berry |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Phytotherapy research : PTR Volume: 22 ISSN: 1099-1573 ISO Abbreviation: Phytother Res Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-05-05 Completed Date: 2008-07-30 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8904486 Medline TA: Phytother Res Country: England |
Other Details:
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Languages: eng Pagination: 675-80 Citation Subset: IM |
Affiliation:
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Department of Cell and Molecular Biology, John A Burns School of Medicine, University of Hawaii at Manoa, 651 Ilalo Street BSB 222, Honolulu, HI 96813, USA. junchen@hawaii.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Bambusa / chemistry* Blotting, Western Cell Line Cell Survival / drug effects Cercopithecus aethiops Fatty Acids, Nonesterified / metabolism Flow Cytometry Mice Palmitic Acid / toxicity* Plant Extracts / chemistry, pharmacology* Proto-Oncogene Proteins c-akt / metabolism Vero Cells |
| Grant Support | |
ID/Acronym/Agency:
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R21 AT003874-01/AT/NCCAM NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Nonesterified; 0/Plant Extracts; 57-10-3/Palmitic Acid; EC 2.7.11.1/Proto-Oncogene Proteins c-akt |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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