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A novel fluorescent method for determination of peroxynitrite using folic acid as a probe.
MedLine Citation:
PMID:  19071757     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A novel method for the determination of peroxynitrite using folic acid as a fluorescent probe is described. The method is based on the oxidation of the reduced, low-fluorescent folic acid by peroxynitrite to produce a high-fluorescent emission product. The fluorescence increase is linearly related to the concentration of peroxynitrite in the range of 3x10(-8) to 5.0x10(-6)molL(-1) with a correlation coefficient of 0.998, and the detection limit is 1x10(-8)molL(-1). Interferences from some metal ions normally seen in biological samples, and also some anions structurally similar to peroxynitrite were studied. The optimal conditions for the detection of peroxynitrite were evaluated.
Authors:
Jun-Chao Huang; De-Jia Li; Jun-Chen Diao; Jie Hou; Jiang-Lan Yuan; Guo-Lin Zou
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Publication Detail:
Type:  Journal Article     Date:  2007-01-20
Journal Detail:
Title:  Talanta     Volume:  72     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2008-12-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  1283-7     Citation Subset:  -    
Affiliation:
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China.
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