| A novel flow bioreactor for in vitro microvascularization. | |
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MedLine Citation:
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PMID: 20170423 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although the importance of fluid flow for proper vascular development and function in vivo is well recognized, microvascular formation in response to flow has not been well evaluated in a three-dimensional (3D) environment in vitro. In this study, we developed a novel 3D in vitro perfusion system that allows direct investigation of the effects of shear stress on the development of microvasculature in vitro. This system utilizes a 3D collagen gel for suspension of vascular cells and mesenchymal stem cells, through which flow is directly perfused. We characterized the flow conditions and demonstrate the impact of flow on the development of microvasculature using a coculture of endothelial cells and mesenchymal stem cells. With the unique ability to apply bulk flow through the collagen gels, and to estimate shear stress within the constructs, this perfusion system provides a flexible platform for developing a controllable biomimetic environment that can be adapted for a variety of investigations of microvascularization. |
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Authors:
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Eun Jung Lee; Laura E Niklason |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Tissue engineering. Part C, Methods Volume: 16 ISSN: 1937-3392 ISO Abbreviation: Tissue Eng Part C Methods Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-29 Completed Date: 2011-02-10 Revised Date: 2011-08-25 |
Medline Journal Info:
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Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: United States |
Other Details:
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Languages: eng Pagination: 1191-200 Citation Subset: IM |
Affiliation:
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Department of Anesthesiology, Yale University, New Haven, CT 06520, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Angiogenesis Inducing Agents
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metabolism Animals Bioreactors* Blotting, Western Cell Proliferation Coculture Techniques Collagen Endothelium, Vascular / cytology Mesenchymal Stem Cells / cytology Microvessels / growth & development* Permeability Rats Tissue Engineering |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL-076485/HL/NHLBI NIH HHS; R01-EB008366/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Angiogenesis Inducing Agents; 9007-34-5/Collagen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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