Document Detail


A novel family of linear plasmids with homology to plasmid pAL2-1 of Podospora anserina.
MedLine Citation:
PMID:  7700237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three recently isolated wild-type strains of the ascomycete Podospora anserina were analyzed for the presence of linear mitochondrial plasmids. In one of these strains, designated Wat, at least 12 distinct plasmid-like elements were identified. From molecular analyses a minimum number of 78 individual linear molecules with proteins bound to their 5' ends was estimated. In addition, the different members of this family of typical linear plasmids were shown to possess a common central region and terminal sequences which differ from one plasmid to another due to the presence of different numbers of a 2.4 kb sequence module. Finally, the pWa6 plasmids share a high degree of sequence similarity with pAL2-1, a linear plasmid previously identified in mitochondria of a long-lived mutant of P.anserina. A mechanism is proposed which explains the generation of these distinct, closely related extra-chromosomal genetic traits.
Authors:
J Hermanns; F Debets; R Hoekstra; H D Osiewacz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  246     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-05-01     Completed Date:  1995-05-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  638-47     Citation Subset:  IM    
Affiliation:
German Cancer Research Center, Department of Molecular Biology of Aging Processes, Heidelberg.
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MeSH Terms
Descriptor/Qualifier:
Ascomycota / genetics*
Base Sequence
DNA Replication / genetics
DNA, Fungal / analysis,  genetics*
DNA, Mitochondrial / analysis,  genetics*
Exodeoxyribonucleases
Models, Genetic
Molecular Sequence Data
Nucleic Acid Conformation
Plasmids / chemistry,  genetics*
Sequence Homology, Nucleic Acid*
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/DNA, Mitochondrial; EC 3.1.-/Exodeoxyribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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