| A novel extracellular cyclic lipopeptide which promotes flagellum-dependent and -independent spreading growth of Serratia marcescens. | |
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MedLine Citation:
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PMID: 1548227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Serrawettin W2, a surface-active exolipid produced by nonpigmented Serratia marcescens NS 25, was examined for its chemical structure and physiological functions. The chemical structure was determined by degradation analyses, infrared spectroscopy, mass spectrometry, and proton magnetic resonance spectroscopy. Serrawettin W2 was shown to be a novel cyclodepsipeptide containing a fatty acid (3-hydroxydecanoic acid) and five amino acids. The peptide was proposed to be D-leucine (N-bonded to the carboxylate of the fatty acid)-L-serine-L-threonine-D-phenylalanine-L-isoleucine (bonded to the 3-hydroxyl group). By examining the effects of isolated serrawettin W2 on serrawettinless mutants, this lipopeptide was shown to be active in the promotion of flagellum-independent spreading growth of the bacteria on a hard agar surface. The parent strain NS 25 formed a giant colony with a self-similar characteristic after incubation for a relatively long time (1 to 2 weeks), similar to other fractal colony-producing strains of S. marcescens (producers of the different serrawettins W1 and W3). On a semisolid medium that permitted flagellum-dependent spreading growth, an external supply of serrawettin W2 accelerated surface translocation of a serrawettinless mutant during a short period (12 h) of observation. In contrast, bacterial translocation in the subsurface space of the semisolid agar was not enhanced by serrawettins. Thus, the extracellular lipids seem to contribute specifically to the surface translocation of the bacteria by exhibiting surfactant activity. |
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Authors:
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T Matsuyama; K Kaneda; Y Nakagawa; K Isa; H Hara-Hotta; I Yano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bacteriology Volume: 174 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1992 Mar |
Date Detail:
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Created Date: 1992-04-23 Completed Date: 1992-04-23 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1769-76 Citation Subset: IM |
Affiliation:
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Department of Bacteriology, Niigata University School of Medicine, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Flagella / physiology Gas Chromatography-Mass Spectrometry Genetic Complementation Test Lipoproteins / chemistry, physiology* Magnetic Resonance Spectroscopy Molecular Sequence Data Peptides, Cyclic / chemistry, physiology* Serratia marcescens / chemistry, growth & development* Surface-Active Agents* / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Lipoproteins; 0/Peptides, Cyclic; 0/Surface-Active Agents; 140909-80-4/serrawettin W2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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