Document Detail

A novel electrochemical biosensor for the detection of uric acid and adenine.
MedLine Citation:
PMID:  16720369     Owner:  NLM     Status:  MEDLINE    
A novel electrochemical biosensor for the detection of uric acid and adenine was prepared based on a gel containing multi-walled carbon nanotubes and room-temperature ionic liquid of 1-octyl-3-methylimidazolium hexafluorophosphate. The electrochemistry of uric acid and adenine was studied in this gel modified electrode. There was a significant two-way electrocatalytic activity upon both oxidation and reduction of uric acid. Similar to a bare glassy carbon electrode, uric acid undergoes a 2e,2H+ oxidation in phosphate buffer in the modified electrode. A diimine, the oxidation product of uric acid, was found to be an unstable intermediate, which was converted by a follow-up hydration reaction to an imine alcohol, with the reaction rate constant of 8.5 +/- 0.3 M(-1).s(-1) according to Nicholson's theory. Under optimum conditions, linear calibration graphs were obtained over the concentration range of 1.0 x 10(-7) M approximately 1.0 x 10(-5) M (uric acid) and 1.0 x 10(-5) M approximately 6.0 x 10(-4) M (adenine). Based on the signal-to-noise ratio of 3, the detection limits of the current technique was found to be as low as 9.0 x 10(-8) M (uric acid) and 2.0 x 10(-6) M (adenine), respectively. This novel biosensor was successfully applied for the assay of uric acid in human urine. Because of its good stability and long-term durability, such a gel modified electrode can provide a simple and easy approach for sensitive detection of uric acid and adenine.
Yifang Zhao; Tianle Ye; Hui Liu; Yuan Kou; Meixian Li; Yuanhua Shao; Zhiwei Zhu; Qiankun Zhuang
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-01
Journal Detail:
Title:  Frontiers in bioscience : a journal and virtual library     Volume:  11     ISSN:  1093-4715     ISO Abbreviation:  Front. Biosci.     Publication Date:  2006  
Date Detail:
Created Date:  2006-05-24     Completed Date:  2006-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9709506     Medline TA:  Front Biosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2976-82     Citation Subset:  IM    
Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China.
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MeSH Terms
Adenine / analysis*
Biosensing Techniques*
Carbon / chemistry
Sensitivity and Specificity
Uric Acid / analysis*
Reg. No./Substance:
69-93-2/Uric Acid; 73-24-5/Adenine; 7440-44-0/Carbon

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