| A novel electrochemical biosensor for the detection of uric acid and adenine. | |
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MedLine Citation:
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PMID: 16720369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel electrochemical biosensor for the detection of uric acid and adenine was prepared based on a gel containing multi-walled carbon nanotubes and room-temperature ionic liquid of 1-octyl-3-methylimidazolium hexafluorophosphate. The electrochemistry of uric acid and adenine was studied in this gel modified electrode. There was a significant two-way electrocatalytic activity upon both oxidation and reduction of uric acid. Similar to a bare glassy carbon electrode, uric acid undergoes a 2e,2H+ oxidation in phosphate buffer in the modified electrode. A diimine, the oxidation product of uric acid, was found to be an unstable intermediate, which was converted by a follow-up hydration reaction to an imine alcohol, with the reaction rate constant of 8.5 +/- 0.3 M(-1).s(-1) according to Nicholson's theory. Under optimum conditions, linear calibration graphs were obtained over the concentration range of 1.0 x 10(-7) M approximately 1.0 x 10(-5) M (uric acid) and 1.0 x 10(-5) M approximately 6.0 x 10(-4) M (adenine). Based on the signal-to-noise ratio of 3, the detection limits of the current technique was found to be as low as 9.0 x 10(-8) M (uric acid) and 2.0 x 10(-6) M (adenine), respectively. This novel biosensor was successfully applied for the assay of uric acid in human urine. Because of its good stability and long-term durability, such a gel modified electrode can provide a simple and easy approach for sensitive detection of uric acid and adenine. |
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Authors:
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Yifang Zhao; Tianle Ye; Hui Liu; Yuan Kou; Meixian Li; Yuanhua Shao; Zhiwei Zhu; Qiankun Zhuang |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-01 |
Journal Detail:
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Title: Frontiers in bioscience : a journal and virtual library Volume: 11 ISSN: 1093-4715 ISO Abbreviation: Front. Biosci. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-05-24 Completed Date: 2006-06-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9709506 Medline TA: Front Biosci Country: United States |
Other Details:
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Languages: eng Pagination: 2976-82 Citation Subset: IM |
Affiliation:
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Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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analysis* Biosensing Techniques* Carbon / chemistry Electrochemistry Electrodes Nanotechnology Sensitivity and Specificity Uric Acid / analysis* |
| Chemical | |
Reg. No./Substance:
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69-93-2/Uric Acid; 73-24-5/Adenine; 7440-44-0/Carbon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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