Document Detail


A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes.
MedLine Citation:
PMID:  17611222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During spermatogenesis, cells coordinate differentiation with the meiotic cell cycle to generate functional gametes. We identified a novel gene, which we named off-schedule (ofs), as being essential for this coordinated control. During the meiotic G(2) phase, Drosophila ofs mutant germ cells do not reach their proper size and fail to execute meiosis or significant differentiation. The accumulation of four cell cycle regulators--Cyclin A, Boule, Twine and Roughex--is altered in these mutants, indicating that ofs reveals a novel branch of the pathway controlling meiosis and differentiation. Ofs is homologous to eukaryotic translation initiation factor eIF4G. The level of ofs expression in spermatocytes is much higher than for the known eIF4G ortholog (known as eIF-4G or eIF4G), suggesting that Ofs substitutes for this protein. Consistent with this, assays for association with mRNA cap complexes, as well as RNA-interference and phenotypic-rescue experiments, demonstrate that Ofs has eIF4G activity. Based on these studies, we speculate that spermatocytes monitor G(2) growth as one means to coordinate the initiation of meiotic division and differentiation.
Authors:
Tina M Franklin-Dumont; Chandrima Chatterjee; Steven A Wasserman; Stephen Dinardo
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-07-04
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  134     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-11     Completed Date:  2007-09-28     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2851-61     Citation Subset:  IM    
Affiliation:
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6048, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Cell Differentiation / genetics*
Cells, Cultured
Cyclin A / metabolism
Drosophila / genetics*
Drosophila Proteins / genetics,  physiology*
Eukaryotic Initiation Factor-4G / genetics,  physiology*
Eye Proteins / genetics
Gene Expression Regulation
Gene Expression Regulation, Developmental
Male
Meiosis / genetics*
Protein Biosynthesis*
Sequence Homology, Amino Acid
Spermatids / cytology
Spermatocytes / cytology*,  growth & development,  metabolism
Tissue Distribution
Grant Support
ID/Acronym/Agency:
GM60804/GM/NIGMS NIH HHS; HD60990/HD/NICHD NIH HHS; T32HD07516/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Cyclin A; 0/Drosophila Proteins; 0/Eukaryotic Initiation Factor-4G; 0/Eye Proteins; 0/off-schedule protein, Drosophila; 0/roughex protein, Drosophila

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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