| A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes. | |
| | |
MedLine Citation:
|
PMID: 17611222 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
During spermatogenesis, cells coordinate differentiation with the meiotic cell cycle to generate functional gametes. We identified a novel gene, which we named off-schedule (ofs), as being essential for this coordinated control. During the meiotic G(2) phase, Drosophila ofs mutant germ cells do not reach their proper size and fail to execute meiosis or significant differentiation. The accumulation of four cell cycle regulators--Cyclin A, Boule, Twine and Roughex--is altered in these mutants, indicating that ofs reveals a novel branch of the pathway controlling meiosis and differentiation. Ofs is homologous to eukaryotic translation initiation factor eIF4G. The level of ofs expression in spermatocytes is much higher than for the known eIF4G ortholog (known as eIF-4G or eIF4G), suggesting that Ofs substitutes for this protein. Consistent with this, assays for association with mRNA cap complexes, as well as RNA-interference and phenotypic-rescue experiments, demonstrate that Ofs has eIF4G activity. Based on these studies, we speculate that spermatocytes monitor G(2) growth as one means to coordinate the initiation of meiotic division and differentiation. |
| | |
Authors:
|
Tina M Franklin-Dumont; Chandrima Chatterjee; Steven A Wasserman; Stephen Dinardo |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-07-04 |
Journal Detail:
|
Title: Development (Cambridge, England) Volume: 134 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 2007 Aug |
Date Detail:
|
Created Date: 2007-07-11 Completed Date: 2007-09-28 Revised Date: 2007-12-03 |
Medline Journal Info:
|
Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
|
Languages: eng Pagination: 2851-61 Citation Subset: IM |
Affiliation:
|
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6048, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Genetically Modified Cell Differentiation / genetics* Cells, Cultured Cyclin A / metabolism Drosophila / genetics* Drosophila Proteins / genetics, physiology* Eukaryotic Initiation Factor-4G / genetics, physiology* Eye Proteins / genetics Gene Expression Regulation Gene Expression Regulation, Developmental Male Meiosis / genetics* Protein Biosynthesis* Sequence Homology, Amino Acid Spermatids / cytology Spermatocytes / cytology*, growth & development, metabolism Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
|
GM60804/GM/NIGMS NIH HHS; HD60990/HD/NICHD NIH HHS; T32HD07516/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cyclin A; 0/Drosophila Proteins; 0/Eukaryotic Initiation Factor-4G; 0/Eye Proteins; 0/off-schedule protein, Drosophila; 0/roughex protein, Drosophila |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Requirement for ERK MAP kinase in mouse preimplantation development.
Next Document: The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development.