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A novel cyclic RGD-containing peptide polymer improves serum-free adhesion of adipose tissue-derived mesenchymal stem cells to bone implant surfaces.
MedLine Citation:
PMID:  23135412     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Seeding of bone implants with mesenchymal stem cells (MSCs) may promote osseointegration and bone regeneration. However, implant material surfaces, such as titanium or bovine bone mineral, fail to support rapid and efficient attachment of MSCs, especially under serum-free conditions that may be desirable when human applications or tightly controlled experiments are envisioned. Here we demonstrate that a branched poly[Lys(Ser(i)-DL-Ala(m))] polymer functionalized with cyclic arginyl-glycyl-aspartate, when immobilized by simple adsorption to tissue culture plastic, surgical titanium alloy (Ti6Al4V), or Bio-Oss(®) bovine bone substitute, significantly accelerates serum-free adhesion and enhances seeding efficiency of human adipose tissue-derived MSCs. Moreover, when exposed to serum-containing osteogenic medium, MSCs survived and differentiated on the peptide-coated scaffolds. In summary, the presented novel polypeptide conjugate can be conveniently used for coating various surfaces, and may find applications whenever quick and efficient seeding of MSCs is required to various scaffolds in the absence of serum.
Authors:
Péter Tátrai; Bernadett Sági; Anna Szigeti; Aron Szepesi; Ildikó Szabó; Szilvia Bősze; Zoltán Kristóf; Károly Markó; Gergely Szakács; István Urbán; Gábor Mező; Ferenc Uher; Katalin Német
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Journal of materials science. Materials in medicine     Volume:  -     ISSN:  1573-4838     ISO Abbreviation:  J Mater Sci Mater Med     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9013087     Medline TA:  J Mater Sci Mater Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Research Center for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
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