Document Detail


A novel complex, RUNX1-MYEF2, represses hematopoietic genes in erythroid cells.
MedLine Citation:
PMID:  22801375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RUNX1 is known to be an essential transcription factor for generating hematopoietic stem cells (HSC), but much less is known about its role in the downstream process of hematopoietic differentiation. RUNX1 has been shown to be part of a large transcription factor complex, together with LDB1, GATA1, TAL1, and ETO2 (N. Meier et al., Development 133:4913-4923, 2006) in erythroid cells. We used a tagging strategy to show that RUNX1 interacts with two novel protein partners, LSD1 and MYEF2, in erythroid cells. MYEF2 is bound in undifferentiated cells and is lost upon differentiation, whereas LSD1 is bound in differentiated cells. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) and microarray expression analysis were used to show that RUNX1 binds approximately 9,000 target sites in erythroid cells and is primarily active in the undifferentiated state. Functional analysis shows that a subset of the target genes is suppressed by RUNX1 via the newly identified partner MYEF2. Knockdown of Myef2 expression in developing zebrafish results in a reduced number of HSC.
Authors:
Boet van Riel; Tibor Pakozdi; Rutger Brouwer; Rui Monteiro; Kapil Tuladhar; Vedran Franke; Jan Christian Bryne; Ruud Jorna; Erik-Jan Rijkers; Wilfred van Ijcken; Charlotte Andrieu-Soler; Jeroen Demmers; Roger Patient; Eric Soler; Boris Lenhard; Frank Grosveld
Related Documents :
22472645 - Effects of donor characteristics and ex vivo expansion on canine mesenchymal stem cell ...
22325385 - In vitro tissue engineering of smooth muscle sheets with peristalsis using a murine ind...
22917235 - Genetically manipulated progenitor/stem cells restore function to the infarcted heart v...
22450675 - The specialized roles of immature and mature dendritic cells in antigen cross-presentat...
21227905 - The embryonic stem cell test as tool to assess structure-dependent teratogenicity: the ...
23845455 - Limited t cell receptor beta variable repertoire responses to esat-6 and cfp-10 in subj...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-16
Journal Detail:
Title:  Molecular and cellular biology     Volume:  32     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-11     Completed Date:  2012-11-26     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3814-22     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Core Binding Factor Alpha 2 Subunit / genetics,  metabolism*
DNA / metabolism
Erythroid Cells / metabolism*
Gene Expression Regulation, Developmental*
Gene Knockdown Techniques
Hematopoiesis*
Mice
Morpholinos / administration & dosage,  genetics
Multiprotein Complexes / metabolism*
Nerve Tissue Proteins / genetics,  metabolism*
Oxidoreductases, N-Demethylating / metabolism*
Protein Binding
Repressor Proteins / genetics,  metabolism*
Zebrafish / embryology
Zebrafish Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
MC_UP_1102/1//Medical Research Council; PG/09/082/28020//British Heart Foundation
Chemical
Reg. No./Substance:
0/Core Binding Factor Alpha 2 Subunit; 0/MYEF2 protein, zebrafish; 0/Morpholinos; 0/Multiprotein Complexes; 0/Myef2 protein, mouse; 0/Nerve Tissue Proteins; 0/Repressor Proteins; 0/Zebrafish Proteins; 9007-49-2/DNA; EC 1.-/Aof2 protein, mouse; EC 1.5.-/Oxidoreductases, N-Demethylating
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Targeting extracellular domains D4 and D7 of vascular endothelial growth factor receptor 2 reveals a...
Next Document:  Fatty acids composition in seeds of the South American glasswort Sarcocornia ambigua.