| A novel, compact disk-like centrifugal microfluidics system for cell lysis and sample homogenization. | |
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MedLine Citation:
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PMID: 17499489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this paper, we present the design and characterization of a novel platform for mechanical cell lysis of even the most difficult to lyse cell types on a micro or nanoscale (maximum 70 microL total volume). The system incorporates a machined plastic circular disk assembly, magnetic field actuated microfluidics, centrifugal cells and tissue homogenizer and centrifugation system. The mechanism of tissue disruption of this novel cell homogenization apparatus derives from the relative motion of ferromagnetic metal disks and grinding matrices in a liquid medium within individual chambers of the disk in the presence of an oscillating magnetic field. The oscillation of the ferromagnetic disks or blades produces mechanical impaction and shear forces capable of disrupting cells within the chamber both by direct action of the blade and by the motion of the surrounding lysis matrix, and by motion induced vortexing of buffer fluid. Glass beads or other grinding media are integrated into each lysis chamber within the disk to enhance the transfer of energy from the oscillating metal blade to the cells. The system also achieves the centrifugal elimination of solids from each liquid sample and allows the elution of clarified supernatants via siphoning into a collection chamber fabricated into the plastic disk assembly. This article describes system design, implementation and validation of proof of concept on two samples--Escherichia coli and Saccharomyces cerevisiae representing model systems for cells that are easy and difficult to lyse, respectively. |
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Authors:
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Horacio Kido; Miodrag Micic; David Smith; Jim Zoval; Jim Norton; Marc Madou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-27 |
Journal Detail:
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Title: Colloids and surfaces. B, Biointerfaces Volume: 58 ISSN: 0927-7765 ISO Abbreviation: Colloids Surf B Biointerfaces Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-05-28 Completed Date: 2007-08-13 Revised Date: 2009-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9315133 Medline TA: Colloids Surf B Biointerfaces Country: Netherlands |
Other Details:
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Languages: eng Pagination: 44-51 Citation Subset: IM |
Affiliation:
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Department of Mechanical and Aerospace Engineering, University of California, Irvine, Irvine, CA 92697-3975, USA. hkido@rotaprep.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Physiological Phenomena* DNA, Bacterial / isolation & purification DNA, Fungal / isolation & purification Electrophoresis, Agar Gel Escherichia coli / cytology Magnetics Microfluidics / instrumentation*, methods Saccharomyces cerevisiae / cytology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Fungal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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