Document Detail

A novel colorimetric potassium sensor based on the substitution of lead from G-quadruplex.
MedLine Citation:
PMID:  23211869     Owner:  NLM     Status:  Publisher    
Potassium ions play diverse roles in biological processes, and abnormal K(+) levels are the hallmarks of diseases. However, the potential clinical application of the developed DNA-based K(+) sensors remains a challenge due to the presence of Pb(2+) in blood samples. In this contribution, a novel colorimetric potassium sensing assay that functions in the presence of Pb(2+) is reported. This approach is based on conformational switching of a hairpin DNA structure to a G-quadruplex. Specifically, the hairpin DNA containing G-rich aptamer T30695 is exposed to successive amounts of Na(+), Pb(2+) and K(+). These cations induce formation of the corresponding metal-stabilized G-quadruplex, which acts as DNAzyme (with hemin as a cofactor) for the catalytic oxidation of ABTS by H(2)O(2). Importantly, studies presented here show that K(+) replaces Pb(2+) from the G-quadruplex to form K(+)-stabilized G-quadruplex, which differ in the catalytic behavior. With Pb(2+)-stabilized G-quadruplex as a probe, a highly sensitive and selective colorimetric detection of K(+) is achieved in the presence of Pb(2+) and excessive Na(+) (140 mM) with the detection limit of 1.9 nM. This system represents the first known DNAzyme-based colorimetric K(+) sensor, which works in the presence of Pb(2+). Finally, the sensor is successfully applied for K(+) detection in a real human serum sample containing Pb(2+).
Huijiao Sun; Xiaohong Li; Yunchao Li; Louzhen Fan; Heinz-Bernhard Kraatz
Related Documents :
24240899 - In vitro short-term bonding performance of zirconia treated with hot acid etching and p...
24664969 - Fusing n-heterocyclic carbenes with carborane anions.
23321219 - New acyclic 1,2,4-triazole-based schiff base hydrazone: synthesis, characterization, sp...
23939069 - Experimental demonstration and observation of a plasmon wave occuring at a gaas-au-gan ...
24377369 - Engineering switchable rotors in molecular crystals with open porosity.
3757019 - Is mucus involved in biocrystallization? study of the intestinal mucus of the sea-water...
24281649 - Interplay between molecule-molecule and molecule-substrate interactions: first-principl...
23468719 - μ-2,5-dihy-droxy-terephthalato-bis-[tri-aqua-(1,10-phenanthroline)zinc] dihy-droxy-ter...
11472149 - Mechanism of beta-hydrogen elimination from square planar iridium(i) alkoxide complexes...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Chemistry, Beijing Normal University, Beijing, 100875, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Halting metastasis through CXCR4 inhibition.
Next Document:  Synthesis, characterization, and self-assembly of linear poly(ethylene oxide)-block-poly(propylene o...