Document Detail


A novel colorimetric biosensor for monitoring and detecting acute toxicity in water.
MedLine Citation:
PMID:  23187797     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This work presents a new colorimetric microorganism biosensor for monitoring and detecting acute toxicity in water, where prussian blue (PB) is used as the colorimetric indicator and E. coli as the model bacterial. In this biosensor, the electron mediator, ferricyanide, accepts electrons from E. coli during respiration to produce ferrocyanide, which subsequently reacts with ferric ions to yield PB, a famous material with a blue color. Since toxicants can inhibit the respiratory activity of E. coli and then reduce the ferrocyanide and consequent PB production, toxicity can be easily detected by measuring the decrease in the production of PB induced by toxicants. Three important toxicants, 3,5-dichlorophenol (DCP), As(3+), Cr(6+) are tested and the detection limits are 3.2, 25, and 3.2 ppm, respectively. Moreover, we could identify the yellow green to dark green color change by naked eye even at concentrations as low as 12.5 ppm for both DCP and Cr(6+). Subsequently, the acute toxicities of groundwater and south lake water are successfully determined by this sensor. This biosensor is rapid, sensitive and cost-effective, and can thus be regarded as a promising biosensor for giving an early warning of acute water toxicity.
Authors:
Junfeng Zhai; Daming Yong; Jing Li; Shaojun Dong
Related Documents :
20641167 - The induction of thrombus generation on nanostructured neutral polymer brush surfaces.
18398447 - Extended peptoids: a new class of oligomers based on aromatic building blocks.
14754217 - Anisotropy of alkyl chains of azobenzene molecules at the air/water interface observed ...
6893277 - Structural reorganizations in lipid bilayer systems: effect of hydration and sterol add...
9532607 - Morphology and surface properties of blends of eudragit rs with different poly(ethylene...
16060157 - Synthesis and characterization of monodispersed polymer/polydiacetylene nanocrystal com...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China. dongsj@ciac.jl.cn.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Role of Intraoperative Radiation Therapy in the Management of Recurrent and Locally Advanced Gyn...
Next Document:  Aerobic biodegradation of propylene glycol by soil bacteria.