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A novel chiral ligand exchange capillary electrophoresis system with amino acid ionic liquid as ligand and its application in screening d-amino-acid oxidase inhibitors.
MedLine Citation:
PMID:  22858807     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A novel amino acid ionic liquid (AAIL) with l-ornithine (l-Orn) as anion was successfully synthesized, and subsequently applied as an available chiral ligand coordinated with Zn(ii) in a chiral ligand exchange capillary electrophoresis (CLE-CE) system for the enantioseparation of dansyl amino acids (Dns-d,l-AAs). The influence of key parameters, such as buffer pH, concentration ratio of Zn(ii) to ligand and complex concentration, was investigated in detail. Eleven pairs of Dns-d,l-AAs enantiomers were baseline separated and three pairs were partly separated under the optimum conditions. For exploring its potential application, the quantitative features of this proposed method were studied. Good linearity (r(2) = 0.999) and favorable repeatability (RSD ≤ 3.4%) were obtained by using Dns-d,l-Met as the test analyte. Finally, this method was employed to investigate the inhibition efficiency of d-amino acid oxidase (DAAO) inhibitors, which may pave a new way for the high-throughput screening of enzyme inhibitors and relevant drug discovery.
Authors:
Xiaoyu Mu; Li Qi; Ying Shen; Haizhi Zhang; Juan Qiao; Huimin Ma
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-3
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun Beiyijie 2#, Beijing, 100190, China. qili@iccas.ac.cn.
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