Document Detail


A novel cell compatible impingement system to study in vitro drug absorption from dry powder aerosol formulations.
MedLine Citation:
PMID:  18771729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modified Astra type multistage liquid impinger (MSLI) with integrated bronchial cell monolayers was used to study deposition and subsequent drug absorption on in vitro models of the human airway epithelial barrier. Inverted cell culture of Calu-3 cells on the bottom side of cell culture filter inserts was integrated into a compendial MSLI. Upside down cultivation did not impair the barrier function, morphology and viability of Calu-3 cells. Size selective deposition with subsequent absorption was studied for three different commercially available dry powder formulations of salbutamol sulphate and budesonide. After deposition without size separation the absorption rates from the aerosol formulations differed but correlated with the size of the carrier lactose particles. However, after deposition in the MSLI, simulating relevant impaction and causing the separation of small drug crystals from the carrier lactose, the absorption rates of the three formulations were identical, confirming the bioequivalence of the three formulations.
Authors:
Michael Bur; Barbara Rothen-Rutishauser; Hanno Huwer; Claus-Michael Lehr
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Publication Detail:
Type:  Journal Article     Date:  2008-08-17
Journal Detail:
Title:  European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V     Volume:  72     ISSN:  1873-3441     ISO Abbreviation:  Eur J Pharm Biopharm     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-19     Completed Date:  2009-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9109778     Medline TA:  Eur J Pharm Biopharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  350-7     Citation Subset:  IM    
Affiliation:
Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbruecken, Germany.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Aerosols*
Cell Line, Tumor
Humans
Pharmacokinetics*
Powders*
Chemical
Reg. No./Substance:
0/Aerosols; 0/Powders

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