| A novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by Bacillus fusiformis CICC 20463, Part II: process optimization. | |
| | |
MedLine Citation:
|
PMID: 19396467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This work optimized the novel biotransformation process of 4'-demethylepipodophyllotoxin (DMEP) into 4'-demethylepipodophyllic acid (DMEPA) by Bacillus fusiformis CICC 20463. Firstly, the biotransformation process was significantly affected by medium composition. 5 g/L of yeast extract and 10 g/L of peptone were optimal for DMEPA production (i.e., 2.81 + or - 0.21 mg/L), while not beneficial for the cell growth of B. fusiformis. This indicated that the biosynthesis of DMEPA was not corresponded well to the cell growth of B. fusiformis. 40 g/L of sucrose was optimal for DMEPA production (i.e., 2.94 + or - 0.17 mg/L), and 3 g/L of NaCl was the best for DMEPA production (i.e., 4.10 + or - 0.18 mg/L). Secondly, the production of DMEPA was significantly enhanced by the control of substrate concentration and culture pH. 100 mg/L of substrate was optimal for DMEPA production (i.e., 6.47 + or - 0.35 mg/L), and DMEPA concentration was enhanced to 38.78 mg/L by controlling culture pH at 9.0 in the stirred-tank bioreactors. The fundamental information obtained in this study provides a simple and efficient way to produce DMEPA by biotransformation. |
| | |
Authors:
|
Ya-Jie Tang; Xiao-Ling Xu; Jian-Jiang Zhong |
Related Documents
:
|
6134147 - Haemoperfusion ineffective for paraquat removal in life-threatening poisoning. 21416037 - Effect of rumex steudelii methanolic root extract on ovarian folliculogenesis and uteri... 20605297 - Arsenic in groundwater and sediment in the mekong river delta, vietnam. 19118857 - Efficacy of controlled-release kmno4 (crp) for controlling dissolved tce plume in groun... 3475987 - Nausea and vomiting after prostaglandins in day case termination of pregnancy. the effi... 12603777 - Influence of escherichia coli endotoxin-induced fever on the pharmacokinetic behavior o... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-25 |
Journal Detail:
|
Title: Bioprocess and biosystems engineering Volume: 33 ISSN: 1615-7605 ISO Abbreviation: Bioprocess Biosyst Eng Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-01-13 Completed Date: 2010-04-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101088505 Medline TA: Bioprocess Biosyst Eng Country: Germany |
Other Details:
|
Languages: eng Pagination: 237-46 Citation Subset: IM |
Affiliation:
|
Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, 430068 Wuhan, China. yajietang@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus
/
metabolism* Bioreactors Biotechnology / methods* Biotransformation* Hydrogen-Ion Concentration Industrial Microbiology / methods* Kinetics Models, Chemical Models, Statistical Nitrogen / chemistry Podophyllotoxin / analogs & derivatives*, chemistry, metabolism Sodium Chloride / chemistry Sucrose / chemistry Surface Properties |
| Chemical | |
Reg. No./Substance:
|
0/4'-demethylepipodophyllic acid; 518-28-5/Podophyllotoxin; 57-50-1/Sucrose; 6559-91-7/4'-demethylepipodophyllotoxin; 7647-14-5/Sodium Chloride; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring.
Next Document: Fatal pulmonary fibrosis after rituximab administration.