Document Detail


A novel binding site for bile acids on human serum albumin.
MedLine Citation:
PMID:  2444264     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Binding sites of bile acids on human serum albumin were studied using various probes: dansylsarcosine (site I probe), 7-anilinocoumarin-4-acetic acid (ACAA, site II probe), 5-dimethylaminonaphthalene-1-sulfonamide (DNSA, site III probe), cis-parinaric acid (probe for fatty acid binding site) and bilirubin. Bile acids competitively inhibited the binding of dansylsarcosine to human serum albumin whereas bile acids enhanced the binding of ACAA, DNSA, cis-parinaric acid and bilirubin. Considering the concentrations of bile acids required to inhibit the binding of dansylsarcosine to human serum albumin, the secondary binding site of bile acids may correspond to site I. Dissociation constants (Kd) of the primary binding sites of lithocholic and chenodeoxycholic acid to human serum albumin were approximately 0.2 and 4 microM, respectively, which was measured by equilibrium dialysis at 37 degrees C. All the bile acids and their sulfates and glucuronides inhibited the binding of chenodeoxycholic acid to human serum albumin. Lithocholic and chenodeoxycholic acid and their sulfates and glucuronides exhibited more inhibition than cholic acid and its conjugates. In conclusion, bile acids may bind to a novel binding site on human serum albumin.
Authors:
H Takikawa; Y Sugiyama; M Hanano; M Kurita; H Yoshida; T Sugimoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  926     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1987-12-14     Completed Date:  1987-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  145-53     Citation Subset:  IM    
Affiliation:
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bile Acids and Salts / blood*
Bilirubin / blood
Binding Sites
Coumarins / blood
Dansyl Compounds / blood
Fatty Acids, Unsaturated / blood
Fluorescent Dyes
Glucuronates / blood
Humans
Kinetics
Oleic Acid
Oleic Acids / blood
Sarcosine / analogs & derivatives,  blood
Serum Albumin / metabolism*
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Coumarins; 0/Dansyl Compounds; 0/Fatty Acids, Unsaturated; 0/Fluorescent Dyes; 0/Glucuronates; 0/Oleic Acids; 0/Serum Albumin; 107-97-1/Sarcosine; 1093-96-5/dansylsarcosine; 112-80-1/Oleic Acid; 1431-39-6/5-dimethylaminonaphthalene-1-sulfonamide; 18427-44-6/parinaric acid; 635-65-4/Bilirubin; 82412-15-5/7-anilinocoumarin-4-acetic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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