Document Detail


A novel biamperometric biosensor for urinary oxalate determination using flow-injection analysis.
MedLine Citation:
PMID:  18804624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A biosensor for determination of oxalate concentration in urine has been developed by immobilisation of oxalate oxidase and peroxidase on the surface of an interdigitated gold electrode. Enzyme immobilisation was performed using BSA and glutaraldehyde. Biamperometric measurements were made in flow conditions both in aqueous oxalate solutions (tested concentration range between 50 microM and 10 mM) and in real urine samples (tested measuring range between 5 and 100 microM). Optimal working conditions were examined for flow-injection analysis, and good correlation was achieved between added oxalate quantity and the one measured by biosensor in urine matrix (R(2)=0.9983). The influence of some interferences (ascorbic acid, uric acid, paracetamol, acetylsalicylic acid) was also studied using biamperometric measurement mode.
Authors:
Stjepan Milardović; Irena Kereković; Marijana Nodilo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-25
Journal Detail:
Title:  Talanta     Volume:  77     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-22     Completed Date:  2008-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  222-8     Citation Subset:  IM    
Affiliation:
Department of General and Inorganic Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, HR-10000 Zagreb, Croatia. stjepan.milardovic@fkit.hr
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / instrumentation*,  methods*
Calibration
Cyanides / urine
Flow Injection Analysis / instrumentation*,  methods*
Hydrogen-Ion Concentration
Indicator Dilution Techniques
Oxalates / urine*
Chemical
Reg. No./Substance:
0/Cyanides; 0/Oxalates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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