Document Detail

A novel, automated method of temperature cycling to produce cryoprecipitate.
MedLine Citation:
PMID:  11239228     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Cryoprecipitate continues to find wide application in transfusion practice. Current AABB standards call for a minimum of 80 units (U) of factor VIII and 150 mg of fibrinogen per bag of cryoprecipitate. However, individual cryoprecipitates can vary greatly in content, with as many as 20 different factors known to affect the yield. STUDY DESIGN AND METHODS: Plasma was processed in a new, rapid, automated device (CryoSeal, Thermogenesis) with computer-controlled temperature cycling to produce cryoprecipitate. RESULTS: In repeat runs (n = 20), the automated procedure yielded a product containing 184 mg of fibrinogen and 158 U of factor VIII in 55 minutes. Additional studies using plasma pools to compare the quality of the machine-generated products to those of traditionally prepared cryoprecipitate showed comparative recoveries of 182 and 187 mg of fibrinogen and 172.1 and 129.7 U of factor VIII and no significant difference in the levels of plasminogen, protein C, or protein S. CONCLUSION: The new system offers an automated method of cryoprecipitate production in which the steps involved in temperature cycling are initiated sequentially, producing within 1 hour a preparation that is equivalent to standard cryoprecipitate.
G Rock; R Berger; J Lange; M Tokessy; D S Palmer; A Giulivi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  41     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-03-12     Completed Date:  2001-04-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  232-5     Citation Subset:  IM    
Ottawa Hospital-Civic Campus and the Canadian Red Cross, Ottawa, Ontario, Canada.
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MeSH Terms
Cryopreservation / instrumentation,  methods*
Factor VIII* / chemistry
Fibrinogen* / chemistry
Plasmapheresis / methods
Reproducibility of Results
Reg. No./Substance:
0/cryoprecipitate coagulum; 9001-27-8/Factor VIII; 9001-32-5/Fibrinogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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