Document Detail


A novel automated assay for malate dehydrogenase isoenzymes.
MedLine Citation:
PMID:  8852359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a new automated method for the determination of malate dehydrogenase (MDH) isoenzymes in serum employing guanidine hydrochloride. Our proposed method showed good reproducibility; within-run precision coefficient of variations (CVs) were less than 2.5 (mean 13.6-42.9 U/L) for total MDH (T-MDH) and less than 6.7% (mean 6.3-23.5 U/L) for mitochondrial MDH (m-MDH) (n = 10). The upper detection limit of the proposed method exhibited good linearity up to 1,000 U/L for both T-MDH and m-MDH. In the proposed m-MDH reagent, the presence of up to 2,000 U/L of cytosolic MDH(c-MDH) activity had no effect on the outcome of m-MDH assay. Results of our proposed method (y) correlated well with those of the electrophoretic method (x) giving the regression equation: y = 1.46 x + 6.87 (N = 30); r = 0.99. Normal concentrations of various anticoagulants and bilirubin did not affect the assay results. Both ascorbic acid and glucose exhibited a slight positive interference with the proposed assay. Clinically, we found that m-MDH activity in serum had greater diagnostic predictive value than T-MDH activity for judging successful outcome of reperfusion therapy; the prognosis was poor when the m-MDH/T-MDH ratio was greater than 20%.
Authors:
H Sugiuchi; Y Uji; Y Shirahase; H Okabe
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical laboratory analysis     Volume:  10     ISSN:  0887-8013     ISO Abbreviation:  J. Clin. Lab. Anal.     Publication Date:  1996  
Date Detail:
Created Date:  1996-12-06     Completed Date:  1996-12-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8801384     Medline TA:  J Clin Lab Anal     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  78-84     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine, Kumamoto University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Coenzymes / analysis,  blood
Electrophoresis
Female
Guanidine
Guanidines
Humans
Hydrogen-Ion Concentration
Isoenzymes / analysis*,  blood
L-Lactate Dehydrogenase / blood,  metabolism
Linear Models
Malate Dehydrogenase / analysis*,  blood
Male
Middle Aged
Myocardial Infarction / enzymology
Oxamic Acid / pharmacology
Prognosis
Protein Denaturation
Reference Values
Reproducibility of Results
Sensitivity and Specificity
Substrate Specificity
Time Factors
Chemical
Reg. No./Substance:
0/Coenzymes; 0/Guanidines; 0/Isoenzymes; 113-00-8/Guanidine; 471-47-6/Oxamic Acid; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.1.1.37/Malate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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