| A novel assay for the biosynthesis of sulphated polysaccharide and its application to studies on the effects of somatomedin on cultured cells. | |
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MedLine Citation:
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PMID: 4786528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. A simple method is described for the determination of small amounts of [(35)S]sulphated polysaccharide with 95-100% recovery in the range from 0.3 to 150mug of polysaccharide. 2. The method is based on precipitation with cetylpyridinium chloride of polysaccharide samples applied to filter paper. It is not significantly disturbed by the presence in the sample of a large excess of inorganic (35)SO(4) (2-). 3. Sulphated glucosamino- and galactosaminoglycans may be determined separately by treatment of the sample with chondroitinase ABC. 4. The method is applicable to the assay of [(35)S]sulphated polysaccharide biosynthesis in cell cultures. A stimulation of sulphate incorporation obeying a linear dose-response curve, was demonstrated in somatomedin-incubated fibroblast and glia cell cultures. 5. The described system provides a new assay method for somatomedin. 6. The stimulatory effect of somatomedin on the synthesis of [(35)S]sulphated polysaccharide appeared to be general, rather than specific, for a particular type of polysaccharide. |
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Authors:
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A Wasteson; K Uthne; B Westermark |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 136 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1973 Dec |
Date Detail:
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Created Date: 1974-06-04 Completed Date: 1974-06-04 Revised Date: 2013-03-26 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1069-74 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured
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drug effects Cetylpyridinium Chemical Precipitation Chondroitin Dose-Response Relationship, Drug Fibroblasts / metabolism Galactosamine / analysis Glucosamine / analysis Humans Lyases / pharmacology Methods Neuroglia / metabolism Polysaccharides / analysis, biosynthesis* Somatomedins / pharmacology* Sulfates / biosynthesis* Sulfur Radioisotopes |
| Chemical | |
Reg. No./Substance:
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0/Polysaccharides; 0/Somatomedins; 0/Sulfates; 0/Sulfur Radioisotopes; 3416-24-8/Glucosamine; 7535-00-4/Galactosamine; 7773-52-6/Cetylpyridinium; 9007-27-6/Chondroitin; EC 4.-/Lyases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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